Spinal autophagy is differently modulated in distinct mouse models of neuropathic pain

Laura Berliocchi, Maria Maiarù, Giuseppe Pasquale Varano, Rossella Russo, Maria Tiziana Corasaniti, Giacinto Bagetta, Cristina Tassorelli

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Autophagy is a homeostatic degradative process essential for basal turnover of long-lived proteins and organelles as well as for removal of dysfunctional cellular components. Dysregulation of the autophagic machinery has been recently associated to several conditions including neurodegenerative diseases and cancer, but only very few studies have investigated its role in pain processing. Results: We previously described autophagy impairment at the spinal cord in the experimental model of neuropathic pain induced by spinal nerve ligation (SNL). In this study, we characterized the main autophagic markers in two other common experimental models of neuropathic pain, the chronic constriction injury (CCI) and the spared nerve injury (SNI). The different modulation of LC3-I, Beclin 1 and p62 suggested that autophagy is differentially affected in the spinal dorsal horn depending on the type of peripheral injury. Confocal analysis of p62 distribution in the spinal dorsal horn indicated its presence mainly in NeuN-positive cell bodies and occasionally in glial processes, thus suggesting a predominant expression in the neuronal compartment. Finally, we investigated the consequences of autophagy impairment on pain behaviour by using the autophagy blocker cloroquine. Intrathecal chloroquine injection in naïve mice induced spinal accumulation of LC3 and p62 paralleled by significant mechanical hypersensitivity thus confirming the block in autophagosome clearance and suggesting the participation of the autophagic process in spinal mechanisms of pain processing. Altogether, our data indicate that spinal autophagy is differentially altered in different experimental pain models of neuropathic pain and that this process may be relevant for pain control.

Original languageEnglish
Article number3
JournalMolecular Pain
Volume11
Issue number1
DOIs
Publication statusPublished - Feb 2 2015

Fingerprint

Autophagy
Neuralgia
Pain
Theoretical Models
Wounds and Injuries
Spinal Injections
Spinal Nerves
Chloroquine
Constriction
Neuroglia
Neurodegenerative Diseases
Organelles
Ligation
Spinal Cord
Hypersensitivity
Neoplasms
Proteins

Keywords

  • Autophagy
  • Beclin 1
  • Chloroquine
  • Chronic constriction injury
  • LC3
  • Neuropathic pain
  • Spared nerve injury
  • Spinal nerve ligation
  • SQSTM1/p62

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Molecular Medicine
  • Cellular and Molecular Neuroscience

Cite this

Berliocchi, L., Maiarù, M., Varano, G. P., Russo, R., Corasaniti, M. T., Bagetta, G., & Tassorelli, C. (2015). Spinal autophagy is differently modulated in distinct mouse models of neuropathic pain. Molecular Pain, 11(1), [3]. https://doi.org/10.1186/1744-8069-11-3

Spinal autophagy is differently modulated in distinct mouse models of neuropathic pain. / Berliocchi, Laura; Maiarù, Maria; Varano, Giuseppe Pasquale; Russo, Rossella; Corasaniti, Maria Tiziana; Bagetta, Giacinto; Tassorelli, Cristina.

In: Molecular Pain, Vol. 11, No. 1, 3, 02.02.2015.

Research output: Contribution to journalArticle

Berliocchi, L, Maiarù, M, Varano, GP, Russo, R, Corasaniti, MT, Bagetta, G & Tassorelli, C 2015, 'Spinal autophagy is differently modulated in distinct mouse models of neuropathic pain', Molecular Pain, vol. 11, no. 1, 3. https://doi.org/10.1186/1744-8069-11-3
Berliocchi L, Maiarù M, Varano GP, Russo R, Corasaniti MT, Bagetta G et al. Spinal autophagy is differently modulated in distinct mouse models of neuropathic pain. Molecular Pain. 2015 Feb 2;11(1). 3. https://doi.org/10.1186/1744-8069-11-3
Berliocchi, Laura ; Maiarù, Maria ; Varano, Giuseppe Pasquale ; Russo, Rossella ; Corasaniti, Maria Tiziana ; Bagetta, Giacinto ; Tassorelli, Cristina. / Spinal autophagy is differently modulated in distinct mouse models of neuropathic pain. In: Molecular Pain. 2015 ; Vol. 11, No. 1.
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