Spinal cord FAAH in normal micturition control and bladder overactivity in awake rats

Claudius Füllhase, Andrea Russo, Fabio Castiglione, Fabio Benigni, Lysanne Campeau, Francesco Montorsi, Christian Gratzke, Arianna Bettiga, Christian Stief, Karl Erik Andersson, Petter Hedlund

Research output: Contribution to journalArticle

Abstract

Purpose: We assessed whether spinal inhibition of the cannabinoid degrading enzyme FAAH would have urodynamic effects in normal rats and rats with bladder overactivity induced by partial urethral obstruction or prostaglandin E2. We also determined the expression of FAAH, and the cannabinoid receptors CB1 and CB2 in the sacral spinal cord. Materials and Methods: We used 44 rats for functional (cystometry) and Western blot experiments. The FAAH inhibitor oleoyl ethyl amide (3 to 300 nmol) was administered intrathecally (subarachnoidally) or intravenously. The expression of FAAH and CB1/CB2 receptors was determined by Western blot. Results: Oleoyl ethyl amide given intrathecally affected micturition in normal rats and rats with bladder overactivity but effects were more pronounced in the latter. In normal rats oleoyl ethyl amide only decreased micturition frequency, while it decreased frequency and bladder pressures in rats with bladder overactivity. Intravenous oleoyl ethyl amide (3 to 300 nmol) had no urodynamic effect. FAAH and CB1/CB2 receptors were expressed in the rat sacral spinal cord. The expression of CB1/CB2 receptors but not FAAH was higher in obstructed than in normal rats. Conclusions: FAAH inhibition in the sacral spinal cord by oleoyl ethyl amide resulted in urodynamic effects in normal rats and rats with bladder overactivity. The spinal endocannabinoid system may be involved in normal micturition control and it appears altered when there is bladder overactivity.

Original languageEnglish
Pages (from-to)2364-2370
Number of pages7
JournalJournal of Urology
Volume189
Issue number6
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Urination
Spinal Cord
Urinary Bladder
Cannabinoid Receptor CB2
Cannabinoid Receptor CB1
Urodynamics
Western Blotting
Urethral Obstruction
Endocannabinoids
Cannabinoids
Dinoprostone
oleoyl ethyl amide
Pressure

Keywords

  • endocannabinoids
  • fatty-acid amide hydrolase
  • overactive
  • urethral obstruction
  • urinary bladder
  • urodynamics

ASJC Scopus subject areas

  • Urology

Cite this

Füllhase, C., Russo, A., Castiglione, F., Benigni, F., Campeau, L., Montorsi, F., ... Hedlund, P. (2013). Spinal cord FAAH in normal micturition control and bladder overactivity in awake rats. Journal of Urology, 189(6), 2364-2370. https://doi.org/10.1016/j.juro.2012.11.165

Spinal cord FAAH in normal micturition control and bladder overactivity in awake rats. / Füllhase, Claudius; Russo, Andrea; Castiglione, Fabio; Benigni, Fabio; Campeau, Lysanne; Montorsi, Francesco; Gratzke, Christian; Bettiga, Arianna; Stief, Christian; Andersson, Karl Erik; Hedlund, Petter.

In: Journal of Urology, Vol. 189, No. 6, 06.2013, p. 2364-2370.

Research output: Contribution to journalArticle

Füllhase, C, Russo, A, Castiglione, F, Benigni, F, Campeau, L, Montorsi, F, Gratzke, C, Bettiga, A, Stief, C, Andersson, KE & Hedlund, P 2013, 'Spinal cord FAAH in normal micturition control and bladder overactivity in awake rats', Journal of Urology, vol. 189, no. 6, pp. 2364-2370. https://doi.org/10.1016/j.juro.2012.11.165
Füllhase, Claudius ; Russo, Andrea ; Castiglione, Fabio ; Benigni, Fabio ; Campeau, Lysanne ; Montorsi, Francesco ; Gratzke, Christian ; Bettiga, Arianna ; Stief, Christian ; Andersson, Karl Erik ; Hedlund, Petter. / Spinal cord FAAH in normal micturition control and bladder overactivity in awake rats. In: Journal of Urology. 2013 ; Vol. 189, No. 6. pp. 2364-2370.
@article{f729eecaef4043f190d7b3da09b54b2c,
title = "Spinal cord FAAH in normal micturition control and bladder overactivity in awake rats",
abstract = "Purpose: We assessed whether spinal inhibition of the cannabinoid degrading enzyme FAAH would have urodynamic effects in normal rats and rats with bladder overactivity induced by partial urethral obstruction or prostaglandin E2. We also determined the expression of FAAH, and the cannabinoid receptors CB1 and CB2 in the sacral spinal cord. Materials and Methods: We used 44 rats for functional (cystometry) and Western blot experiments. The FAAH inhibitor oleoyl ethyl amide (3 to 300 nmol) was administered intrathecally (subarachnoidally) or intravenously. The expression of FAAH and CB1/CB2 receptors was determined by Western blot. Results: Oleoyl ethyl amide given intrathecally affected micturition in normal rats and rats with bladder overactivity but effects were more pronounced in the latter. In normal rats oleoyl ethyl amide only decreased micturition frequency, while it decreased frequency and bladder pressures in rats with bladder overactivity. Intravenous oleoyl ethyl amide (3 to 300 nmol) had no urodynamic effect. FAAH and CB1/CB2 receptors were expressed in the rat sacral spinal cord. The expression of CB1/CB2 receptors but not FAAH was higher in obstructed than in normal rats. Conclusions: FAAH inhibition in the sacral spinal cord by oleoyl ethyl amide resulted in urodynamic effects in normal rats and rats with bladder overactivity. The spinal endocannabinoid system may be involved in normal micturition control and it appears altered when there is bladder overactivity.",
keywords = "endocannabinoids, fatty-acid amide hydrolase, overactive, urethral obstruction, urinary bladder, urodynamics",
author = "Claudius F{\"u}llhase and Andrea Russo and Fabio Castiglione and Fabio Benigni and Lysanne Campeau and Francesco Montorsi and Christian Gratzke and Arianna Bettiga and Christian Stief and Andersson, {Karl Erik} and Petter Hedlund",
year = "2013",
month = "6",
doi = "10.1016/j.juro.2012.11.165",
language = "English",
volume = "189",
pages = "2364--2370",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Spinal cord FAAH in normal micturition control and bladder overactivity in awake rats

AU - Füllhase, Claudius

AU - Russo, Andrea

AU - Castiglione, Fabio

AU - Benigni, Fabio

AU - Campeau, Lysanne

AU - Montorsi, Francesco

AU - Gratzke, Christian

AU - Bettiga, Arianna

AU - Stief, Christian

AU - Andersson, Karl Erik

AU - Hedlund, Petter

PY - 2013/6

Y1 - 2013/6

N2 - Purpose: We assessed whether spinal inhibition of the cannabinoid degrading enzyme FAAH would have urodynamic effects in normal rats and rats with bladder overactivity induced by partial urethral obstruction or prostaglandin E2. We also determined the expression of FAAH, and the cannabinoid receptors CB1 and CB2 in the sacral spinal cord. Materials and Methods: We used 44 rats for functional (cystometry) and Western blot experiments. The FAAH inhibitor oleoyl ethyl amide (3 to 300 nmol) was administered intrathecally (subarachnoidally) or intravenously. The expression of FAAH and CB1/CB2 receptors was determined by Western blot. Results: Oleoyl ethyl amide given intrathecally affected micturition in normal rats and rats with bladder overactivity but effects were more pronounced in the latter. In normal rats oleoyl ethyl amide only decreased micturition frequency, while it decreased frequency and bladder pressures in rats with bladder overactivity. Intravenous oleoyl ethyl amide (3 to 300 nmol) had no urodynamic effect. FAAH and CB1/CB2 receptors were expressed in the rat sacral spinal cord. The expression of CB1/CB2 receptors but not FAAH was higher in obstructed than in normal rats. Conclusions: FAAH inhibition in the sacral spinal cord by oleoyl ethyl amide resulted in urodynamic effects in normal rats and rats with bladder overactivity. The spinal endocannabinoid system may be involved in normal micturition control and it appears altered when there is bladder overactivity.

AB - Purpose: We assessed whether spinal inhibition of the cannabinoid degrading enzyme FAAH would have urodynamic effects in normal rats and rats with bladder overactivity induced by partial urethral obstruction or prostaglandin E2. We also determined the expression of FAAH, and the cannabinoid receptors CB1 and CB2 in the sacral spinal cord. Materials and Methods: We used 44 rats for functional (cystometry) and Western blot experiments. The FAAH inhibitor oleoyl ethyl amide (3 to 300 nmol) was administered intrathecally (subarachnoidally) or intravenously. The expression of FAAH and CB1/CB2 receptors was determined by Western blot. Results: Oleoyl ethyl amide given intrathecally affected micturition in normal rats and rats with bladder overactivity but effects were more pronounced in the latter. In normal rats oleoyl ethyl amide only decreased micturition frequency, while it decreased frequency and bladder pressures in rats with bladder overactivity. Intravenous oleoyl ethyl amide (3 to 300 nmol) had no urodynamic effect. FAAH and CB1/CB2 receptors were expressed in the rat sacral spinal cord. The expression of CB1/CB2 receptors but not FAAH was higher in obstructed than in normal rats. Conclusions: FAAH inhibition in the sacral spinal cord by oleoyl ethyl amide resulted in urodynamic effects in normal rats and rats with bladder overactivity. The spinal endocannabinoid system may be involved in normal micturition control and it appears altered when there is bladder overactivity.

KW - endocannabinoids

KW - fatty-acid amide hydrolase

KW - overactive

KW - urethral obstruction

KW - urinary bladder

KW - urodynamics

UR - http://www.scopus.com/inward/record.url?scp=84877601035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877601035&partnerID=8YFLogxK

U2 - 10.1016/j.juro.2012.11.165

DO - 10.1016/j.juro.2012.11.165

M3 - Article

C2 - 23219540

AN - SCOPUS:84877601035

VL - 189

SP - 2364

EP - 2370

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 6

ER -