Sponge-Like Dressings Based on the Association of Chitosan and Sericin for the Treatment of Chronic Skin Ulcers. II. Loading of the Hemoderivative Platelet Lysate

Michela Mori, Silvia Rossi, Franca Ferrari, Maria C. Bonferoni, Giuseppina Sandri, Federica Riva, Marika Tenci, Claudia Del Fante, Giovanni Nicoletti, Carla Caramella

Research output: Contribution to journalArticle

Abstract

Platelet lysate (PL) was loaded into dressings based on chitosan glutamate (CSG) low and high molecular weight, sericin (Ser), and glycine (Gly). A synergic effect of Ser and PL on fibroblast proliferation was proved in vitro. Two different PL loading approaches were considered: the first provided to prepare dressings by freeze-drying a mixture of PL and CSG/Gly/Ser solution, the second approach consisted in the extemporarily loading of PL in the CSG/Gly/Ser freeze-dried dressings. As for the first approach, PL loading did not produce any variation in dressing mechanical properties. Such dressings absorbed a high amount (about 8-fold of dry weight) of phosphate-buffered saline (fluid mimicking wound exudate), forming a gel with pseudoplastic and elastic properties. Platelet-derived growth factor AB assay indicated that neither freeze-drying nor the excipients alter PL growth factor content. As for the second approach, mechanical and rheological properties of the gel formed upon PL absorption enabled to choose a PL loading of about 90 μL/cm2. Upon contact with fibroblasts, all PL loaded formulations increased the number not only of viable cells but also of those in the proliferative phase. Histological studies effected on human skin strips pointed out the positive effect of PL loaded dressings on dermal matrix reconstruction.

Original languageEnglish
Pages (from-to)1188-1195
Number of pages8
JournalJournal of Pharmaceutical Sciences
Volume105
Issue number3
DOIs
Publication statusPublished - Mar 1 2016

Keywords

  • biomaterials
  • cell culture
  • chitosan
  • skin
  • wound healing

ASJC Scopus subject areas

  • Pharmaceutical Science

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