Spontaneous apoptosis of thymocytes is uncoupled with progression through the cell cycle

C. Pellicciari, M. G. Bottone, V. Schaack, S. Barni, A. A. Manfredi

Research output: Contribution to journalArticle

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Abstract

Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same cell population of death and proliferation is thus apparent in developing thymocytes. Using dual-parameter cytometric techniques to evaluate in single cells the amount of DNA versus light- scattering values, we found that spontaneous thymocyte apoptosis occurs with similar frequency in all the cycle phases, whereas apoptosis induced by the anti-topoisomerase-II, etoposide (which is the consequence of irreversible DNA damage), takes place with higher frequency in S and G2 phases (i.e., in those cycle phases in which DNA is subjected to torsional constraints). The capability of thymocytes to enter apoptosis was also monitored by digesting DNA in situ with DNase I (a nuclease that cleaves DNA mimicking the nuclear damage common to most apoptotic suicides). We also show that endonuclease- mediated DNA digestion occurs to a similar extent in cells with different DNA contents, i.e., in cycle phases in which the superstructural organization of chromatin is markedly different.

Original languageEnglish
Pages (from-to)370-377
Number of pages8
JournalExperimental Cell Research
Volume229
Issue number2
DOIs
Publication statusPublished - Dec 15 1996

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Thymocytes
Cell Cycle
Apoptosis
Deoxyribonuclease I
DNA
Ki-67 Antigen
Type II DNA Topoisomerase
Deoxyribonucleases
G2 Phase
Proliferating Cell Nuclear Antigen
Etoposide
S Phase
Suicide
Thymus Gland
DNA Damage
Chromatin
Digestion
Cell Death
Cell Proliferation
Lymphocytes

ASJC Scopus subject areas

  • Cell Biology

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Spontaneous apoptosis of thymocytes is uncoupled with progression through the cell cycle. / Pellicciari, C.; Bottone, M. G.; Schaack, V.; Barni, S.; Manfredi, A. A.

In: Experimental Cell Research, Vol. 229, No. 2, 15.12.1996, p. 370-377.

Research output: Contribution to journalArticle

Pellicciari, C. ; Bottone, M. G. ; Schaack, V. ; Barni, S. ; Manfredi, A. A. / Spontaneous apoptosis of thymocytes is uncoupled with progression through the cell cycle. In: Experimental Cell Research. 1996 ; Vol. 229, No. 2. pp. 370-377.
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