Spontaneous calcium oscillations regulate human cardiac progenitor cell growth

João Ferreira-Martins, Carlos Rondon-Clavo, Derin Tugal, Justin A. Korn, Roberto Rizzi, Maria Elena Padin-Iruegas, Sergio Ottolenghi, Antonella De Angelis, Konrad Urbanek, Noriko Ide-Iwata, Domenico D'Amario, Toru Hosoda, Annarosa Leri, Jan Kajstura, Piero Anversa, Marcello Rota

Research output: Contribution to journalArticle

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Abstract

RATIONALE:: The adult heart possesses a pool of progenitor cells stored in myocardial niches, but the mechanisms involved in the activation of this cell compartment are currently unknown. OBJECTIVE:: Ca promotes cell growth raising the possibility that changes in intracellular Ca initiate division of c-kit-positive human cardiac progenitor cells (hCPCs) and determine their fate. METHODS AND RESULTS:: Ca oscillations were identified in hCPCs and these events occurred independently from coupling with cardiomyocytes or the presence of extracellular Ca. These findings were confirmed in the heart of transgenic mice in which enhanced green fluorescent protein was under the control of the c-kit promoter. Ca oscillations in hCPCs were regulated by the release of Ca from the endoplasmic reticulum through activation of inositol 1,4,5-triphosphate receptors (IP3Rs) and the reuptake of Ca by the sarco-/endoplasmic reticulum Ca pump (SERCA). IP3Rs and SERCA were highly expressed in hCPCs, whereas ryanodine receptors were not detected. Although Na-Ca exchanger, store-operated Ca channels and plasma membrane Ca pump were present and functional in hCPCs, they had no direct effects on Ca oscillations. Conversely, Ca oscillations and their frequency markedly increased with ATP and histamine which activated purinoceptors and histamine-1 receptors highly expressed in hCPCs. Importantly, Ca oscillations in hCPCs were coupled with the entry of cells into the cell cycle and 5-bromodeoxyuridine incorporation. Induction of Ca oscillations in hCPCs before their intramyocardial delivery to infarcted hearts was associated with enhanced engraftment and expansion of these cells promoting the generation of a large myocyte progeny. CONCLUSION:: IP3R-mediated Ca mobilization control hCPC growth and their regenerative potential.

Original languageEnglish
Pages (from-to)764-774
Number of pages11
JournalCirculation Research
Volume105
Issue number8
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Calcium Signaling
Stem Cells
Growth
Endoplasmic Reticulum
Inositol 1,4,5-Trisphosphate Receptors
Purinergic Receptors
Histamine Receptors
Ryanodine Receptor Calcium Release Channel
Bromodeoxyuridine
Cardiac Myocytes
Muscle Cells
Histamine
Transgenic Mice
Cell Cycle
Adenosine Triphosphate
Cell Membrane

Keywords

  • Calcium oscillations
  • Cell growth
  • Human cardiac progenitor cells

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Ferreira-Martins, J., Rondon-Clavo, C., Tugal, D., Korn, J. A., Rizzi, R., Padin-Iruegas, M. E., ... Rota, M. (2009). Spontaneous calcium oscillations regulate human cardiac progenitor cell growth. Circulation Research, 105(8), 764-774. https://doi.org/10.1161/CIRCRESAHA.109.206698

Spontaneous calcium oscillations regulate human cardiac progenitor cell growth. / Ferreira-Martins, João; Rondon-Clavo, Carlos; Tugal, Derin; Korn, Justin A.; Rizzi, Roberto; Padin-Iruegas, Maria Elena; Ottolenghi, Sergio; De Angelis, Antonella; Urbanek, Konrad; Ide-Iwata, Noriko; D'Amario, Domenico; Hosoda, Toru; Leri, Annarosa; Kajstura, Jan; Anversa, Piero; Rota, Marcello.

In: Circulation Research, Vol. 105, No. 8, 10.2009, p. 764-774.

Research output: Contribution to journalArticle

Ferreira-Martins, J, Rondon-Clavo, C, Tugal, D, Korn, JA, Rizzi, R, Padin-Iruegas, ME, Ottolenghi, S, De Angelis, A, Urbanek, K, Ide-Iwata, N, D'Amario, D, Hosoda, T, Leri, A, Kajstura, J, Anversa, P & Rota, M 2009, 'Spontaneous calcium oscillations regulate human cardiac progenitor cell growth', Circulation Research, vol. 105, no. 8, pp. 764-774. https://doi.org/10.1161/CIRCRESAHA.109.206698
Ferreira-Martins J, Rondon-Clavo C, Tugal D, Korn JA, Rizzi R, Padin-Iruegas ME et al. Spontaneous calcium oscillations regulate human cardiac progenitor cell growth. Circulation Research. 2009 Oct;105(8):764-774. https://doi.org/10.1161/CIRCRESAHA.109.206698
Ferreira-Martins, João ; Rondon-Clavo, Carlos ; Tugal, Derin ; Korn, Justin A. ; Rizzi, Roberto ; Padin-Iruegas, Maria Elena ; Ottolenghi, Sergio ; De Angelis, Antonella ; Urbanek, Konrad ; Ide-Iwata, Noriko ; D'Amario, Domenico ; Hosoda, Toru ; Leri, Annarosa ; Kajstura, Jan ; Anversa, Piero ; Rota, Marcello. / Spontaneous calcium oscillations regulate human cardiac progenitor cell growth. In: Circulation Research. 2009 ; Vol. 105, No. 8. pp. 764-774.
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AU - Ferreira-Martins, João

AU - Rondon-Clavo, Carlos

AU - Tugal, Derin

AU - Korn, Justin A.

AU - Rizzi, Roberto

AU - Padin-Iruegas, Maria Elena

AU - Ottolenghi, Sergio

AU - De Angelis, Antonella

AU - Urbanek, Konrad

AU - Ide-Iwata, Noriko

AU - D'Amario, Domenico

AU - Hosoda, Toru

AU - Leri, Annarosa

AU - Kajstura, Jan

AU - Anversa, Piero

AU - Rota, Marcello

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N2 - RATIONALE:: The adult heart possesses a pool of progenitor cells stored in myocardial niches, but the mechanisms involved in the activation of this cell compartment are currently unknown. OBJECTIVE:: Ca promotes cell growth raising the possibility that changes in intracellular Ca initiate division of c-kit-positive human cardiac progenitor cells (hCPCs) and determine their fate. METHODS AND RESULTS:: Ca oscillations were identified in hCPCs and these events occurred independently from coupling with cardiomyocytes or the presence of extracellular Ca. These findings were confirmed in the heart of transgenic mice in which enhanced green fluorescent protein was under the control of the c-kit promoter. Ca oscillations in hCPCs were regulated by the release of Ca from the endoplasmic reticulum through activation of inositol 1,4,5-triphosphate receptors (IP3Rs) and the reuptake of Ca by the sarco-/endoplasmic reticulum Ca pump (SERCA). IP3Rs and SERCA were highly expressed in hCPCs, whereas ryanodine receptors were not detected. Although Na-Ca exchanger, store-operated Ca channels and plasma membrane Ca pump were present and functional in hCPCs, they had no direct effects on Ca oscillations. Conversely, Ca oscillations and their frequency markedly increased with ATP and histamine which activated purinoceptors and histamine-1 receptors highly expressed in hCPCs. Importantly, Ca oscillations in hCPCs were coupled with the entry of cells into the cell cycle and 5-bromodeoxyuridine incorporation. Induction of Ca oscillations in hCPCs before their intramyocardial delivery to infarcted hearts was associated with enhanced engraftment and expansion of these cells promoting the generation of a large myocyte progeny. CONCLUSION:: IP3R-mediated Ca mobilization control hCPC growth and their regenerative potential.

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