Bone marrow cells from a patient with pancytopenia and a lymphoproliferative disorder of large granular lymphocytes (LGL) were cultured and tested for their hemopoietic colony-forming potential. Neither erythroid nor granulocyte-macrophage colony formation could be obtained from unfractionated, or LGL-depleted bone marrow cell preparations. However, a spontaneous growth of lymphoid colonies was observed after culturing LGL-depleted (T3-) bone marrow cell suspensions for 25 days. Pooled colonies expanded with recombinent interleukin-2 yielded a population composed predominantly of mature T cells (T3+, Leu 6+). These findings suggest that some (T3-) T cell precursors may mature in the bone marrow and that, in our patient, LGL may have exerted a suppressor effect on this maturational process.
|Number of pages||8|
|Journal||Natural Immunity and Cell Growth Regulation|
|Publication status||Published - 1987|
ASJC Scopus subject areas
- Clinical Biochemistry