Spontaneous in vitro production of virus-specific antibody by lymphocytes from HIV-infected subjects

Alberto Amadori, Anita De Rossi, Georgine P. Faulkner-Valle, Luigi Chieco-Bianchi

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro synthesis of IgG directed against HIV components was detected by ELISA and Western blot assay of lymphocyte culture supernatants. Lymphocytes from HIV-infected individuals spontaneously produced antibody against HIV proteins very early in culture, suggesting in vivo activation of HIV-specific antibody-forming cells. The frequency of circulating B cells spontaneously secreting HIV-specific IgG was very high in some cases, but spontaneous HIV-specific antibody synthesis was not accompanied by polyclonal reactivation of B-cell clones of different specificity. The pattern of specificity of the anti-HIV antibody produced in vitro did not reflect the serum pattern consistently. These findings indicate a new approach potentially useful for the study of the immunobiology of HIV infection. The possible implications of the in vitro production of HIV-specific antibody for the diagnosis, prognosis and clinical management of this infection are also discussed.

Original languageEnglish
Pages (from-to)342-351
Number of pages10
JournalClinical Immunology and Immunopathology
Volume46
Issue number3
DOIs
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

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