Spontaneous shaker rat mutant - A new model for X-linked tremor/ataxia

Karla P. Figueroa, Sharan Paul, Tito Calì, Raffaele Lopreiato, Sukanya Karan, Martina Frizzarin, Darren Ames, Ginevra Zanni, Marisa Brini, Warunee Dansithong, Brett Milash, Daniel R. Scoles, Ernesto Carafoli, Stefan M. Pulst

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The shaker rat is an X-linked recessive spontaneous model of progressive Purkinje cell (PC) degeneration exhibiting a shaking ataxia and wide stance. Generation of Wistar Furth (WF)/Brown Norwegian (BN) F1 hybrids and genetic mapping of F2 sib-sib offspring using polymorphic markers narrowed the candidate gene region to 26 Mbp denoted by the last recombinant genetic marker DXRat21 at 133 Mbp to qter (the end of the long arm). In the WF background, the shaker mutation has complete penetrance, results in a stereotypic phenotype and there is a narrow window for age of disease onset; by contrast, the F2 hybrid phenotypewas more varied, with a later age of onset and likely non-penetrance of the mutation. By deep RNA-sequencing, five variants were found in the candidate region; four were novel without known annotation. One of the variants caused an arginine (R) to cysteine (C) change at codon 35 of the ATPase, Ca2+ transporting, plasma membrane 3 (Atp2b3) gene encoding PMCA3 that has high expression in the cerebellum. The variant was well supported by hundreds of overlapping reads, and was found in 100% of all affected replicas and 0% of the wild-type (WT) replicas. The mutation segregated with disease in all affected animals and the amino acid change was found in an evolutionarily conserved region of PMCA3. Despite strong genetic evidence for pathogenicity, in vitro analyses of PMCA3R35C function did not show any differences to WT PMCA3. Because Atp2b3 mutation leads to congenital ataxia in humans, the identified Atp2b3 missense change in the shaker rat presents a good candidate for the shaker rat phenotype based on genetic criteria, but cannot yet be considered a definite pathogenic variant owing to lack of functional changes.

Original languageEnglish
Pages (from-to)553-562
Number of pages10
JournalDMM Disease Models and Mechanisms
Volume9
Issue number5
DOIs
Publication statusPublished - May 1 2016

Fingerprint

Tremor
Ataxia
Rats
Mutation
Age of Onset
Gene encoding
Calcium-Transporting ATPases
Cell membranes
RNA Sequence Analysis
Phenotype
High-Throughput Nucleotide Sequencing
Cysteine
Arginine
Penetrance
Purkinje Cells
Animals
Genes
Genetic Markers
Codon
RNA

Keywords

  • Ataxia
  • Atp2b3
  • PMCA3
  • Shaker
  • X-linked

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine (miscellaneous)
  • Immunology and Microbiology (miscellaneous)
  • Neuroscience (miscellaneous)

Cite this

Figueroa, K. P., Paul, S., Calì, T., Lopreiato, R., Karan, S., Frizzarin, M., ... Pulst, S. M. (2016). Spontaneous shaker rat mutant - A new model for X-linked tremor/ataxia. DMM Disease Models and Mechanisms, 9(5), 553-562. https://doi.org/10.1242/dmm.022848

Spontaneous shaker rat mutant - A new model for X-linked tremor/ataxia. / Figueroa, Karla P.; Paul, Sharan; Calì, Tito; Lopreiato, Raffaele; Karan, Sukanya; Frizzarin, Martina; Ames, Darren; Zanni, Ginevra; Brini, Marisa; Dansithong, Warunee; Milash, Brett; Scoles, Daniel R.; Carafoli, Ernesto; Pulst, Stefan M.

In: DMM Disease Models and Mechanisms, Vol. 9, No. 5, 01.05.2016, p. 553-562.

Research output: Contribution to journalArticle

Figueroa, KP, Paul, S, Calì, T, Lopreiato, R, Karan, S, Frizzarin, M, Ames, D, Zanni, G, Brini, M, Dansithong, W, Milash, B, Scoles, DR, Carafoli, E & Pulst, SM 2016, 'Spontaneous shaker rat mutant - A new model for X-linked tremor/ataxia', DMM Disease Models and Mechanisms, vol. 9, no. 5, pp. 553-562. https://doi.org/10.1242/dmm.022848
Figueroa KP, Paul S, Calì T, Lopreiato R, Karan S, Frizzarin M et al. Spontaneous shaker rat mutant - A new model for X-linked tremor/ataxia. DMM Disease Models and Mechanisms. 2016 May 1;9(5):553-562. https://doi.org/10.1242/dmm.022848
Figueroa, Karla P. ; Paul, Sharan ; Calì, Tito ; Lopreiato, Raffaele ; Karan, Sukanya ; Frizzarin, Martina ; Ames, Darren ; Zanni, Ginevra ; Brini, Marisa ; Dansithong, Warunee ; Milash, Brett ; Scoles, Daniel R. ; Carafoli, Ernesto ; Pulst, Stefan M. / Spontaneous shaker rat mutant - A new model for X-linked tremor/ataxia. In: DMM Disease Models and Mechanisms. 2016 ; Vol. 9, No. 5. pp. 553-562.
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