Sporulation: An alternative way to recover recombinant proteins from Bacillus subtilis

G. Maurizi, P. Bossu, D. Boraschi, E. Ulisse, A. Tagliabue, P. Ruggiero

Research output: Contribution to journalArticle

Abstract

A recombinant strain of Bacillus subtilis engineered for endocellular expression of human interleukin-1 receptor antagonist (IL-1ra) was subjected to sporulation. The recombinant protein was recovered from the sporulation supernatant in quantities, purity, and activity comparable with those obtained from a traditional cell lysate. Thus, exploitation of this natural mechanism of autolysis could overcome problems of intact protein recovery related to the cell disruption step.

Original languageEnglish
Pages (from-to)197-200
Number of pages4
JournalBiotechnology and Bioengineering
Volume48
Issue number3
DOIs
Publication statusPublished - Nov 5 1995

Fingerprint

Recombinant proteins
Interleukin-1 Receptors
Bacilli
Bacillus subtilis
Recombinant Proteins
Proteins
Autolysis
Recovery

Keywords

  • B. subtilis
  • cell lysis
  • recombinant protein purification
  • sporulation

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Sporulation : An alternative way to recover recombinant proteins from Bacillus subtilis. / Maurizi, G.; Bossu, P.; Boraschi, D.; Ulisse, E.; Tagliabue, A.; Ruggiero, P.

In: Biotechnology and Bioengineering, Vol. 48, No. 3, 05.11.1995, p. 197-200.

Research output: Contribution to journalArticle

Maurizi, G. ; Bossu, P. ; Boraschi, D. ; Ulisse, E. ; Tagliabue, A. ; Ruggiero, P. / Sporulation : An alternative way to recover recombinant proteins from Bacillus subtilis. In: Biotechnology and Bioengineering. 1995 ; Vol. 48, No. 3. pp. 197-200.
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