Sporulation: An alternative way to recover recombinant proteins from Bacillus subtilis

G. Maurizi, P. Bossu, D. Boraschi, E. Ulisse, A. Tagliabue, P. Ruggiero

Research output: Contribution to journalArticlepeer-review

Abstract

A recombinant strain of Bacillus subtilis engineered for endocellular expression of human interleukin-1 receptor antagonist (IL-1ra) was subjected to sporulation. The recombinant protein was recovered from the sporulation supernatant in quantities, purity, and activity comparable with those obtained from a traditional cell lysate. Thus, exploitation of this natural mechanism of autolysis could overcome problems of intact protein recovery related to the cell disruption step.

Original languageEnglish
Pages (from-to)197-200
Number of pages4
JournalBiotechnology and Bioengineering
Volume48
Issue number3
DOIs
Publication statusPublished - Nov 5 1995

Keywords

  • B. subtilis
  • cell lysis
  • recombinant protein purification
  • sporulation

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Fingerprint Dive into the research topics of 'Sporulation: An alternative way to recover recombinant proteins from Bacillus subtilis'. Together they form a unique fingerprint.

Cite this