Abstract
The Schistosoma mansoni fatty acid binding protein (FABP), Sm14, is a vaccine candidate against, S. mansoni and F. hepatica. Previously, we demonstrated the importance of a correct fold to achieve protection in immunized animals after cercariae challenge [[10]. C.R.R. Ramos, R.C.R. Figueredo, T.A. Pertinhez, M.M. Vilar, A.L.T.O. Nascimento, M. Tendler, I. Raw, A. Spisni, P.L. Ho, Gene structure and M20T polymorphism of the Schistosoma mansoni Sm14 fatty acid-binding protein: structural, functional and immunoprotection analysis. J. Biol. Chem. 278 (2003) 12745-12751.]. Here we show that the reduction of vaccine efficacy over time is due to protein dimerization and subsequent aggregation. We produced the mutants Sm14-M20(C62S) and Sm14-M20(C62V) that, as expected, did not dimerize in SDS-PAGE. Molecular dynamics calculations and unfolding experiments highlighted a higher structural stability of these mutants with respect to the wild-type. In addition, we found that the mutated proteins, after thermal denaturation, refolded to their active native molecular architecture as proved by the recovery of the fatty acid binding ability. Sm14-M20(C62V) turned out to be the more stable form over time, providing the basis to determine the first 3D solution structure of a Sm14 protein in its apo-form. Overall, Sm14-M20(C62V) possesses an improved structural stability over time, an essential feature to preserve its immunization capability and, in experimentally immunized animals, it exhibits a protection effect against S. mansoni cercariae infections comparable to the one obtained with the wild-type protein. These facts indicate this protein as a good lead molecule for large-scale production and for developing an effective Sm14 based anti-helminthes vaccine.
| Original language | English |
|---|---|
| Pages (from-to) | 655-662 |
| Number of pages | 8 |
| Journal | Biochimica et Biophysica Acta - Proteins and Proteomics |
| Volume | 1794 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2009 |
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Keywords
- Fatty-acid binding protein
- Nuclear Magnetic Resonance
- Schistosoma mansoni
- Schistosomiasis
- Sm14
- Vaccine
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Analytical Chemistry
- Molecular Biology
Cite this
Stability improvement of the fatty acid binding protein Sm14 from S. mansoni by Cys replacement : Structural and functional characterization of a vaccine candidate. / Ramos, Celso R R; Spisni, Alberto; Oyama, Sérgio; Sforça, Mauricio L.; Ramos, Henrique R.; Vilar, Mônica M.; Alves, Adriana C.; Figueredo, Rita C R; Tendler, Míriam; Zanchin, Nilson I T; Pertinhez, Thelma A.; Ho, Paulo Lee.
In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1794, No. 4, 04.2009, p. 655-662.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stability improvement of the fatty acid binding protein Sm14 from S. mansoni by Cys replacement
T2 - Structural and functional characterization of a vaccine candidate
AU - Ramos, Celso R R
AU - Spisni, Alberto
AU - Oyama, Sérgio
AU - Sforça, Mauricio L.
AU - Ramos, Henrique R.
AU - Vilar, Mônica M.
AU - Alves, Adriana C.
AU - Figueredo, Rita C R
AU - Tendler, Míriam
AU - Zanchin, Nilson I T
AU - Pertinhez, Thelma A.
AU - Ho, Paulo Lee
PY - 2009/4
Y1 - 2009/4
N2 - The Schistosoma mansoni fatty acid binding protein (FABP), Sm14, is a vaccine candidate against, S. mansoni and F. hepatica. Previously, we demonstrated the importance of a correct fold to achieve protection in immunized animals after cercariae challenge [[10]. C.R.R. Ramos, R.C.R. Figueredo, T.A. Pertinhez, M.M. Vilar, A.L.T.O. Nascimento, M. Tendler, I. Raw, A. Spisni, P.L. Ho, Gene structure and M20T polymorphism of the Schistosoma mansoni Sm14 fatty acid-binding protein: structural, functional and immunoprotection analysis. J. Biol. Chem. 278 (2003) 12745-12751.]. Here we show that the reduction of vaccine efficacy over time is due to protein dimerization and subsequent aggregation. We produced the mutants Sm14-M20(C62S) and Sm14-M20(C62V) that, as expected, did not dimerize in SDS-PAGE. Molecular dynamics calculations and unfolding experiments highlighted a higher structural stability of these mutants with respect to the wild-type. In addition, we found that the mutated proteins, after thermal denaturation, refolded to their active native molecular architecture as proved by the recovery of the fatty acid binding ability. Sm14-M20(C62V) turned out to be the more stable form over time, providing the basis to determine the first 3D solution structure of a Sm14 protein in its apo-form. Overall, Sm14-M20(C62V) possesses an improved structural stability over time, an essential feature to preserve its immunization capability and, in experimentally immunized animals, it exhibits a protection effect against S. mansoni cercariae infections comparable to the one obtained with the wild-type protein. These facts indicate this protein as a good lead molecule for large-scale production and for developing an effective Sm14 based anti-helminthes vaccine.
AB - The Schistosoma mansoni fatty acid binding protein (FABP), Sm14, is a vaccine candidate against, S. mansoni and F. hepatica. Previously, we demonstrated the importance of a correct fold to achieve protection in immunized animals after cercariae challenge [[10]. C.R.R. Ramos, R.C.R. Figueredo, T.A. Pertinhez, M.M. Vilar, A.L.T.O. Nascimento, M. Tendler, I. Raw, A. Spisni, P.L. Ho, Gene structure and M20T polymorphism of the Schistosoma mansoni Sm14 fatty acid-binding protein: structural, functional and immunoprotection analysis. J. Biol. Chem. 278 (2003) 12745-12751.]. Here we show that the reduction of vaccine efficacy over time is due to protein dimerization and subsequent aggregation. We produced the mutants Sm14-M20(C62S) and Sm14-M20(C62V) that, as expected, did not dimerize in SDS-PAGE. Molecular dynamics calculations and unfolding experiments highlighted a higher structural stability of these mutants with respect to the wild-type. In addition, we found that the mutated proteins, after thermal denaturation, refolded to their active native molecular architecture as proved by the recovery of the fatty acid binding ability. Sm14-M20(C62V) turned out to be the more stable form over time, providing the basis to determine the first 3D solution structure of a Sm14 protein in its apo-form. Overall, Sm14-M20(C62V) possesses an improved structural stability over time, an essential feature to preserve its immunization capability and, in experimentally immunized animals, it exhibits a protection effect against S. mansoni cercariae infections comparable to the one obtained with the wild-type protein. These facts indicate this protein as a good lead molecule for large-scale production and for developing an effective Sm14 based anti-helminthes vaccine.
KW - Fatty-acid binding protein
KW - Nuclear Magnetic Resonance
KW - Schistosoma mansoni
KW - Schistosomiasis
KW - Sm14
KW - Vaccine
UR - http://www.scopus.com/inward/record.url?scp=61649110721&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61649110721&partnerID=8YFLogxK
U2 - 10.1016/j.bbapap.2008.12.010
DO - 10.1016/j.bbapap.2008.12.010
M3 - Article
C2 - 19150418
AN - SCOPUS:61649110721
VL - 1794
SP - 655
EP - 662
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
SN - 1570-9639
IS - 4
ER -