Stability of endogenous GHB in vitreous humor vs peripheral blood in dead bodies

Francesco Paolo Busardò, Giulio Mannocchi, Raffaele Giorgetti, Manuela Pellegrini, Giovanni Baglio, Simona Zaami, Enrico Marinelli, Simona Pichini

Research output: Contribution to journalArticle

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Abstract

For the first time, the stability of GHB was tested in post-mortem peripheral blood and vitreous humor samples, collected from 22 dead bodies at two different times: at the external body examination at the place of death and then during autopsy. An ad hoc method for the detection and quantification of GHB in vitreous humor by gas chromatography coupled to mass spectrometry (GC-MS) was developed and validated, with a good linearity between 0.1 and 50μg/mL (r2=0.991) and a precision and accuracy always better than 10% and an analytical recovery higher than 90%. The geometric mean of GHB concentration in the 22 peripheral blood samples at t0was: 3.6μg/mL (95% CI: 2.3-5.9μg/mL) and at t1it was 7.4μg/mL (95% CI: 5.0-10.9μg/mL); that of GHB in the 22 vitreous humor at t0was: 2.5μg/mL (95% CI: 1.5-4.1μg/mL) and at t1it was 3.0μg/mL (95% CI: 1.9-4.8μg/mL). There was no significant difference between the GHB concentrations in vitreous humor and peripheral blood at t0in all the samples (p>0.10). Conversely at t1, the increase of GHB in the peripheral blood was significantly increased by a 102% (range: 86-120%) (p<0.001 vs t0), while in the vitreous humor only a slight increase by 19% was observed (range: 16-21%) (p>0.05 vs t0). Finally at t1, GHB values in the two matrices were statistically different, being that of peripheral blood higher (p<0.01). This study demonstrated the usefulness of vitreous humor as a more stable alternative matrix in comparison to peripheral blood for the post-mortem determination of endogenous GHB.

Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalForensic Science International
Volume274
DOIs
Publication statusPublished - May 2017

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Vitreous Body
Gas Chromatography
Autopsy
Mass Spectrometry

Keywords

  • Gas chromatography mass spectrometry
  • GHB
  • Peripheral blood
  • Post-mortem stability
  • Vitreous humor

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Stability of endogenous GHB in vitreous humor vs peripheral blood in dead bodies. / Busardò, Francesco Paolo; Mannocchi, Giulio; Giorgetti, Raffaele; Pellegrini, Manuela; Baglio, Giovanni; Zaami, Simona; Marinelli, Enrico; Pichini, Simona.

In: Forensic Science International, Vol. 274, 05.2017, p. 64-69.

Research output: Contribution to journalArticle

Busardò, Francesco Paolo ; Mannocchi, Giulio ; Giorgetti, Raffaele ; Pellegrini, Manuela ; Baglio, Giovanni ; Zaami, Simona ; Marinelli, Enrico ; Pichini, Simona. / Stability of endogenous GHB in vitreous humor vs peripheral blood in dead bodies. In: Forensic Science International. 2017 ; Vol. 274. pp. 64-69.
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abstract = "For the first time, the stability of GHB was tested in post-mortem peripheral blood and vitreous humor samples, collected from 22 dead bodies at two different times: at the external body examination at the place of death and then during autopsy. An ad hoc method for the detection and quantification of GHB in vitreous humor by gas chromatography coupled to mass spectrometry (GC-MS) was developed and validated, with a good linearity between 0.1 and 50μg/mL (r2=0.991) and a precision and accuracy always better than 10{\%} and an analytical recovery higher than 90{\%}. The geometric mean of GHB concentration in the 22 peripheral blood samples at t0was: 3.6μg/mL (95{\%} CI: 2.3-5.9μg/mL) and at t1it was 7.4μg/mL (95{\%} CI: 5.0-10.9μg/mL); that of GHB in the 22 vitreous humor at t0was: 2.5μg/mL (95{\%} CI: 1.5-4.1μg/mL) and at t1it was 3.0μg/mL (95{\%} CI: 1.9-4.8μg/mL). There was no significant difference between the GHB concentrations in vitreous humor and peripheral blood at t0in all the samples (p>0.10). Conversely at t1, the increase of GHB in the peripheral blood was significantly increased by a 102{\%} (range: 86-120{\%}) (p<0.001 vs t0), while in the vitreous humor only a slight increase by 19{\%} was observed (range: 16-21{\%}) (p>0.05 vs t0). Finally at t1, GHB values in the two matrices were statistically different, being that of peripheral blood higher (p<0.01). This study demonstrated the usefulness of vitreous humor as a more stable alternative matrix in comparison to peripheral blood for the post-mortem determination of endogenous GHB.",
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AU - Busardò, Francesco Paolo

AU - Mannocchi, Giulio

AU - Giorgetti, Raffaele

AU - Pellegrini, Manuela

AU - Baglio, Giovanni

AU - Zaami, Simona

AU - Marinelli, Enrico

AU - Pichini, Simona

N1 - Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

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N2 - For the first time, the stability of GHB was tested in post-mortem peripheral blood and vitreous humor samples, collected from 22 dead bodies at two different times: at the external body examination at the place of death and then during autopsy. An ad hoc method for the detection and quantification of GHB in vitreous humor by gas chromatography coupled to mass spectrometry (GC-MS) was developed and validated, with a good linearity between 0.1 and 50μg/mL (r2=0.991) and a precision and accuracy always better than 10% and an analytical recovery higher than 90%. The geometric mean of GHB concentration in the 22 peripheral blood samples at t0was: 3.6μg/mL (95% CI: 2.3-5.9μg/mL) and at t1it was 7.4μg/mL (95% CI: 5.0-10.9μg/mL); that of GHB in the 22 vitreous humor at t0was: 2.5μg/mL (95% CI: 1.5-4.1μg/mL) and at t1it was 3.0μg/mL (95% CI: 1.9-4.8μg/mL). There was no significant difference between the GHB concentrations in vitreous humor and peripheral blood at t0in all the samples (p>0.10). Conversely at t1, the increase of GHB in the peripheral blood was significantly increased by a 102% (range: 86-120%) (p<0.001 vs t0), while in the vitreous humor only a slight increase by 19% was observed (range: 16-21%) (p>0.05 vs t0). Finally at t1, GHB values in the two matrices were statistically different, being that of peripheral blood higher (p<0.01). This study demonstrated the usefulness of vitreous humor as a more stable alternative matrix in comparison to peripheral blood for the post-mortem determination of endogenous GHB.

AB - For the first time, the stability of GHB was tested in post-mortem peripheral blood and vitreous humor samples, collected from 22 dead bodies at two different times: at the external body examination at the place of death and then during autopsy. An ad hoc method for the detection and quantification of GHB in vitreous humor by gas chromatography coupled to mass spectrometry (GC-MS) was developed and validated, with a good linearity between 0.1 and 50μg/mL (r2=0.991) and a precision and accuracy always better than 10% and an analytical recovery higher than 90%. The geometric mean of GHB concentration in the 22 peripheral blood samples at t0was: 3.6μg/mL (95% CI: 2.3-5.9μg/mL) and at t1it was 7.4μg/mL (95% CI: 5.0-10.9μg/mL); that of GHB in the 22 vitreous humor at t0was: 2.5μg/mL (95% CI: 1.5-4.1μg/mL) and at t1it was 3.0μg/mL (95% CI: 1.9-4.8μg/mL). There was no significant difference between the GHB concentrations in vitreous humor and peripheral blood at t0in all the samples (p>0.10). Conversely at t1, the increase of GHB in the peripheral blood was significantly increased by a 102% (range: 86-120%) (p<0.001 vs t0), while in the vitreous humor only a slight increase by 19% was observed (range: 16-21%) (p>0.05 vs t0). Finally at t1, GHB values in the two matrices were statistically different, being that of peripheral blood higher (p<0.01). This study demonstrated the usefulness of vitreous humor as a more stable alternative matrix in comparison to peripheral blood for the post-mortem determination of endogenous GHB.

KW - Gas chromatography mass spectrometry

KW - GHB

KW - Peripheral blood

KW - Post-mortem stability

KW - Vitreous humor

U2 - 10.1016/j.forsciint.2016.12.025

DO - 10.1016/j.forsciint.2016.12.025

M3 - Article

C2 - 28065555

VL - 274

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JO - Forensic Science International

JF - Forensic Science International

SN - 0379-0738

ER -