Stabilization of cellular mRNAs and up-regulation of proteins by oligoribonucleotides homologous to the Bcl2 adenine-uridine rich element motif

Annamaria Bevilacqua, Laura Ghisolfi, Sara Franzi, Giovanna Maresca, Roberto Gherzi, Sergio Capaccioli, Angelo Nicolin, Gianfranco Canti

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Adenine-uridine rich elements (AREs) play an important role in modulating mRNA stability, being the target site of many ARE-binding proteins (AUBPs) that are involved in the decay process. Three 26-mer 2′-O-methyl oligoribonucleotides (ORNs) homologous to the core region of ARE of bcl2 mRNA have been studied for decoy-aptamer activity in UV cross-linking assays. Sense-oriented ORNs competed with the ARE motif for the interaction with both destabilizing and stabilizing AUBPs in cell-free systems and in cell lines. Moreover, ORNs induced mRNA stabilization and up-regulated both Bcl2 mRNA and protein levels in the cells. Bcl2 ORNs stabilized other ARE-containing transcripts and up-regulated their expression. These results indicate that Bcl2 ORNs compete for AUBP-ARE interactions independently of ARE class and suggest that in the cell, the default labile status of ARE-containing mRNAs depends on the combined interaction of such transcripts with destabilizing AUBPs.

Original languageEnglish
Pages (from-to)531-538
Number of pages8
JournalMolecular Pharmacology
Volume71
Issue number2
DOIs
Publication statusPublished - Feb 2007

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Oligoribonucleotides
Uridine
Adenine
Up-Regulation
Messenger RNA
Proteins
Cell-Free System
RNA Stability
Carrier Proteins
Cell Line

ASJC Scopus subject areas

  • Pharmacology

Cite this

Stabilization of cellular mRNAs and up-regulation of proteins by oligoribonucleotides homologous to the Bcl2 adenine-uridine rich element motif. / Bevilacqua, Annamaria; Ghisolfi, Laura; Franzi, Sara; Maresca, Giovanna; Gherzi, Roberto; Capaccioli, Sergio; Nicolin, Angelo; Canti, Gianfranco.

In: Molecular Pharmacology, Vol. 71, No. 2, 02.2007, p. 531-538.

Research output: Contribution to journalArticle

Bevilacqua, Annamaria ; Ghisolfi, Laura ; Franzi, Sara ; Maresca, Giovanna ; Gherzi, Roberto ; Capaccioli, Sergio ; Nicolin, Angelo ; Canti, Gianfranco. / Stabilization of cellular mRNAs and up-regulation of proteins by oligoribonucleotides homologous to the Bcl2 adenine-uridine rich element motif. In: Molecular Pharmacology. 2007 ; Vol. 71, No. 2. pp. 531-538.
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