Stabilization of the T-state of ferrous human adult haemoglobin by chlorpromazine and trifluoperazine

Paolo Ascenzi, Alberto Bertollini, Massimo Coletta, Antonio Lucacchini

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In the present study, the effect of the neuroleptics chlorpromazine (2-chloro-N,N-dimethyl-10H-phenothiazine-10-propanamine) and trifluoperazine {10-[3-(4-methylpiperazin-1-yl)-propyl]-2-(trifluoromethyl)-10H -phenothiazine} on the EPR-spectroscopic properties of ferrous human adult nitrosylated haemoglobin (HbNO) is reported. Addition of the two drugs to HbNO shifted the conformational equilibrium from the high- to the low-affinity form of the ligated tetramer, as observed for 2,3-D-glycerate bisphosphate, the physiological modulator of haemoglobin action. The effect of chlorpromarine and trifluoperazine on the EPR-spectroscopic properties of HbNO was enhanced by inositol hexakisphosphate. The binding of neuroleptics to ferrous human adult haemoglobin may represent an important undesirable side effect. In fact, oxygen affinity for ferrous human adult haemoglobin decreases on increasing chlorpromazine and trifluoperazine concentration. In addition, red blood cells may act as neuroleptic scavengers.

Original languageEnglish
Pages (from-to)185-187
Number of pages3
JournalBiotechnology and Applied Biochemistry
Volume30
Issue number2
Publication statusPublished - 1999

Fingerprint

Trifluoperazine
Hemoglobin
Chlorpromazine
Hemoglobins
Stabilization
Antipsychotic Agents
Paramagnetic resonance
2,3-Diphosphoglycerate
Phytic Acid
Modulators
Blood
Erythrocytes
Cells
Oxygen
Pharmaceutical Preparations
glycosylated-nitric oxide complex hemoglobin A

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Stabilization of the T-state of ferrous human adult haemoglobin by chlorpromazine and trifluoperazine. / Ascenzi, Paolo; Bertollini, Alberto; Coletta, Massimo; Lucacchini, Antonio.

In: Biotechnology and Applied Biochemistry, Vol. 30, No. 2, 1999, p. 185-187.

Research output: Contribution to journalArticle

Ascenzi, P, Bertollini, A, Coletta, M & Lucacchini, A 1999, 'Stabilization of the T-state of ferrous human adult haemoglobin by chlorpromazine and trifluoperazine', Biotechnology and Applied Biochemistry, vol. 30, no. 2, pp. 185-187.
Ascenzi, Paolo ; Bertollini, Alberto ; Coletta, Massimo ; Lucacchini, Antonio. / Stabilization of the T-state of ferrous human adult haemoglobin by chlorpromazine and trifluoperazine. In: Biotechnology and Applied Biochemistry. 1999 ; Vol. 30, No. 2. pp. 185-187.
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