Stabilization of the T-state of ferrous human adult haemoglobin by chlorpromazine and trifluoperazine

Paolo Ascenzi, Alberto Bertollini, Massimo Coletta, Antonio Lucacchini

Research output: Contribution to journalArticle

Abstract

In the present study, the effect of the neuroleptics chlorpromazine (2-chloro-N,N-dimethyl-10H-phenothiazine-10-propanamine) and trifluoperazine {10-[3-(4-methylpiperazin-1-yl)-propyl]-2-(trifluoromethyl)-10H -phenothiazine} on the EPR-spectroscopic properties of ferrous human adult nitrosylated haemoglobin (HbNO) is reported. Addition of the two drugs to HbNO shifted the conformational equilibrium from the high- to the low-affinity form of the ligated tetramer, as observed for 2,3-D-glycerate bisphosphate, the physiological modulator of haemoglobin action. The effect of chlorpromarine and trifluoperazine on the EPR-spectroscopic properties of HbNO was enhanced by inositol hexakisphosphate. The binding of neuroleptics to ferrous human adult haemoglobin may represent an important undesirable side effect. In fact, oxygen affinity for ferrous human adult haemoglobin decreases on increasing chlorpromazine and trifluoperazine concentration. In addition, red blood cells may act as neuroleptic scavengers.

Original languageEnglish
Pages (from-to)185-187
Number of pages3
JournalBiotechnology and Applied Biochemistry
Volume30
Issue number2
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Fingerprint Dive into the research topics of 'Stabilization of the T-state of ferrous human adult haemoglobin by chlorpromazine and trifluoperazine'. Together they form a unique fingerprint.

  • Cite this