Stabilization of the T-state of human hemoglobin by proflavine, an antiseptic drug

Paolo Ascenzi, Marco Colasanti, Mauro Fasano, Alberto Bertollini

Research output: Contribution to journalArticle

Abstract

The effect of proflavine (3,6-diaminoacridine), an antiseptic drug, on the spectroscopic and oxygen binding properties of ferrous human adult hemoglobin (Hb) has been investigated. Upon binding of proflavine to the nitric oxide derivative of ferrous human adult hemoglobin (HbNO), the X-band EPR spectrum displays the characteristics which have been attributed to the T-state of the ligated tetramer. In parallel, oxygen affinity for the deoxygenated derivative of ferrous human adult Hb decreases in the presence of proflavine. The effect of proflavine on the spectroscopic and ligand binding properties of ferrous human adult Hb is reminiscent that of 2,3-D-glycerate bisphosphate, the physiological modulator of Hb action.

Original languageEnglish
Pages (from-to)991-995
Number of pages5
JournalBiochemistry and Molecular Biology International
Volume47
Issue number6
Publication statusPublished - Jun 1999

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Keywords

  • Allosteric effector
  • Antiseptic drug
  • Ferrous human adult hemoglobin
  • Oxygen binding properties
  • Proflavine
  • Spectroscopic properties

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

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