Stable knockdown of microRNA in vivo by lentiviral vectors

Bernhard Gentner, Giulia Schira, Alice Giustacchini, Mario Amendola, Brian D. Brown, Maurilio Ponzoni, Luigi Naldini

Research output: Contribution to journalArticle

245 Citations (Scopus)

Abstract

Studying microRNA function in vivo requires genetic strategies to generate loss-of-function phenotypes. We used lentiviral vectors to stably and specifically knock down microRNA by overexpressing microRNA target sequences from polymerase II promoters. These vectors effectively inhibited regulation of reporter constructs and natural microRNA targets. We used bone marrow reconstitution with hematopoietic stem cells stably overexpressing miR-223 target sequence to phenocopy the genetic miR-223 knockout mouse, indicating robust interference of microRNA function in vivo.

Original languageEnglish
Pages (from-to)63-66
Number of pages4
JournalNature Methods
Volume6
Issue number1
DOIs
Publication statusPublished - 2009

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MicroRNAs
Hematopoietic Stem Cells
Stem cells
Knockout Mice
Bone
Bone Marrow
Phenotype

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Stable knockdown of microRNA in vivo by lentiviral vectors. / Gentner, Bernhard; Schira, Giulia; Giustacchini, Alice; Amendola, Mario; Brown, Brian D.; Ponzoni, Maurilio; Naldini, Luigi.

In: Nature Methods, Vol. 6, No. 1, 2009, p. 63-66.

Research output: Contribution to journalArticle

Gentner, Bernhard ; Schira, Giulia ; Giustacchini, Alice ; Amendola, Mario ; Brown, Brian D. ; Ponzoni, Maurilio ; Naldini, Luigi. / Stable knockdown of microRNA in vivo by lentiviral vectors. In: Nature Methods. 2009 ; Vol. 6, No. 1. pp. 63-66.
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