Stable non-covalent large area patterning of inert Teflon-AF surface: A new approach to multiscale cell guidance

Francesco Valle, Beatrice Chelli, Michele Bianchi, Pierpaolo Greco, Eva Bystrenova, Ilaria Tonazzini, Fabio Biscarini

Research output: Contribution to journalArticlepeer-review

Abstract

Micro-and nano-patterning of cell adhesion proteins is demonstrated to direct the growth of neural cells, viz. human neuroblastoma SHSY5Y, at precise positions on a strongly antifouling substrate of technolological interest. We adopt a soft-lithographic approach with oxygen plasma modified PDMS stamps to pattern human laminin on Teflon-AF films. These patterns are based on the interplay of capillary forces within the stamp and non-covalent intermolecular and surface interactions. Remarkably, they remain stable for several days upon cell culture conditions. The fabrication of substrates with adjacent antifouling and adhesion-promoting regions allows us to reach absolute spatial control in the positioning of neuroblastoma cells on the Teflon-AF films. This patterning approach of a technologicallyrelevant substrate can be of interest in tissue engineering and biosensing.

Original languageEnglish
JournalAdvanced Engineering Materials
Volume12
Issue number6
DOIs
Publication statusPublished - Jun 2010

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

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