Standardization of activated protein C resistance testing: Effect of residual platelets in frozen plasmas assessed by commercial and home-made methods

Armando Tripodi, Carla Valsecchi, Veena Chantarangkul, Tullia Battaglioli, Pier Mannuccio Mannucci

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated 42 plasmas prepared at different centrifugation speeds with three activated protein C (APC) resistance methods. The APC ratio for fresh platelet-poor plasma declined significantly after freezing and thawing. This effect was more evident with the original method (average reduction 11.3%) than with either the home-made (3.8%) or the modified method (3.2%). No significant decrease in the APC ratio was observed after freezing and thawing of platelet-free plasmas from the same patients. When frozen platelet-poor plasma was centrifuged at high speed after thawing and before testing, there was no significant decrease in the APC ratio, in comparison with fresh platelet-poor plasma using the home-made and modified methods.

Original languageEnglish
Pages (from-to)825-828
Number of pages4
JournalBritish Journal of Haematology
Volume120
Issue number5
DOIs
Publication statusPublished - Mar 1 2003

Keywords

  • Activated protein C (APC) resistance
  • Platelets
  • Standardization
  • Thrombophilia

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Standardization of activated protein C resistance testing: Effect of residual platelets in frozen plasmas assessed by commercial and home-made methods'. Together they form a unique fingerprint.

Cite this