STANDARDIZZAZIONE DEL DOSAGGIO DEL COLESTEROLO HDL: RISULTATI OTTENUTI DAL LABORATORIO #504 DEL CHOLESTEROL REFERENCE METHOD LABORATORY NETWORK

Translated title of the contribution: Standardization of HDL-cholesterol measurements: Results obtained by laboratory #504 of Cholesterol Reference Method Laboratory Network

A. Modenese, L. Baldo, E. De Giorgi, F. Ceriotti, C. A. Ferrero, A. Carobene

Research output: Contribution to journalArticle

Abstract

HDL-cholesterol (HDL-C) value is an essential component in evaluation of dislipidemias. The Centers for Disease Control (CDC Atlanta), after a preliminary multicentre evaluation, selected dextran sulfate as precipitating agent for HDL-C determination. The protocol studied implies the measuring of 1 mL of serum sample with 100 μL of precipitant (dextran-sulfate) (50000 D) 10 g/L, MgC12 0.35 mol/L, NaN3 0.5 g/L) and the cholesterol measurement on the supernatant using the Abell-Kendall reference procedure. Our laboratory togheter with other 12 laboratories of Cholesterol Reference Method Laboratory Network (CRMLN), participated to a two phases standardization procedure. In the first phase 3 materials were analyzed in quadruplicate in four different runs, in the second phase 4 materials were analyzed in duplicate in eight different runs. CV obtained by our laboratory were always below 3% with bias <3%. The selected dextran sulfate method is transferable (interlaboratory CV <3%. Precipitating step reproducibility is very high and doesn't affect the overall precision. The results obtained by all the network laboratories presented bias <5% from CDC ultracentrifugative method. With these good results we can foresee certification activity within few months.

Translated title of the contributionStandardization of HDL-cholesterol measurements: Results obtained by laboratory #504 of Cholesterol Reference Method Laboratory Network
Original languageItalian
Pages (from-to)205-211
Number of pages7
JournalGiornale Italiano di Chimica Clinica
Volume20
Issue number4
Publication statusPublished - 1995

ASJC Scopus subject areas

  • Clinical Biochemistry

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