HDL-cholesterol (HDL-C) value is an essential component in evaluation of dislipidemias. The Centers for Disease Control (CDC Atlanta), after a preliminary multicentre evaluation, selected dextran sulfate as precipitating agent for HDL-C determination. The protocol studied implies the measuring of 1 mL of serum sample with 100 μL of precipitant (dextran-sulfate) (50000 D) 10 g/L, MgC12 0.35 mol/L, NaN3 0.5 g/L) and the cholesterol measurement on the supernatant using the Abell-Kendall reference procedure. Our laboratory togheter with other 12 laboratories of Cholesterol Reference Method Laboratory Network (CRMLN), participated to a two phases standardization procedure. In the first phase 3 materials were analyzed in quadruplicate in four different runs, in the second phase 4 materials were analyzed in duplicate in eight different runs. CV obtained by our laboratory were always below 3% with bias <3%. The selected dextran sulfate method is transferable (interlaboratory CV <3%. Precipitating step reproducibility is very high and doesn't affect the overall precision. The results obtained by all the network laboratories presented bias <5% from CDC ultracentrifugative method. With these good results we can foresee certification activity within few months.
|Translated title of the contribution||Standardization of HDL-cholesterol measurements: Results obtained by laboratory #504 of Cholesterol Reference Method Laboratory Network|
|Number of pages||7|
|Journal||Giornale Italiano di Chimica Clinica|
|Publication status||Published - 1995|
ASJC Scopus subject areas
- Clinical Biochemistry