Standardization with synthetic 22-kDa monomer human growth hormone reduces discrepancies between two monoclonal immunoradiometric assay kits

G. Banfi, M. Marinelli, M. Pontillo, P. Bonini

Research output: Contribution to journalArticlepeer-review

Abstract

Discrepancies among different methods for assaying human growth hormone have been described in various studies. The two major sources of discordant results are the heterogeneity of the antibodies and the different standardization bases used by the assay manufacturers. We propose standardizing assays with 22-kDa biosynthetic monomer human growth hormone diluted with the diluents supplied by the kit manufacturers. In a study of two monoclonal immunoradiometric assays (Hybritech, specific for the 22-kDa monomer; Sorin, recognizing also a 20-kDa variant hormone), standardization with 22-kDa monomer human growth hormone reduced by 63% the differences in results for 44 serum samples from children. The use of 22-kDa human growth hormone as a common standard, highly pure and easily available in large quantities, could help limit the interpretative problems in growth diagnostics.

Original languageEnglish
Pages (from-to)2107-2110
Number of pages4
JournalClinical Chemistry
Volume38
Issue number10
Publication statusPublished - 1992

Keywords

  • somatotropin
  • source of
  • variation

ASJC Scopus subject areas

  • Clinical Biochemistry

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