Abstract
OBJECTIVE: To evaluate interobserver reproducibility of histologic grade in endometrial adenocarcinomas of endometrioid type (EC), to assess the relationships between nuclear grade and the amount of argyrophilic nucleolar organizer region (AgNOR) proteins and to determine the prognostic value of AgNOR proteins and the main clinicopathologic parameters. STUDY DESIGN: Architectural and nuclear grading were independently assessed by two pathologists in 64 formalin-fixed, paraffin-embedded surgical samples of EC obtained from an equal number of patients (age range, 38-84 years; mean, 63.5). Interobserver agreement was determined using the κ statistic; discrepant cases were reviewed, and a consensus was reached. Standardized AgNOR analysis was performed according to the guidelines of the Committee on AgNOR Quantification, measuring the mean area of AgNORs per nucleus (NORA) by an image analysis system. RESULTS: The κ values for interobserver agreement were substantial for architectural grading and moderate for nuclear grading. When NORA values were compared to the nuclear grade assessed by different observers, the most significant linear correlation (r = .713, P 4.212 μm2), while, by Cox multivariate analysis, AgNOR quantity emerged as an independent prognostic variable. CONCLUSION: Use of standardized AgNOR analysis may be an additional and objective tool in the assessment of histologic grade as well as a reliable method of determining prognosis in EC.
| Original language | English |
|---|---|
| Pages (from-to) | 31-40 |
| Number of pages | 10 |
| Journal | Analytical and Quantitative Cytology and Histology |
| Volume | 23 |
| Issue number | 1 |
| Publication status | Published - 2001 |
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Keywords
- Endometrial neoplasms
- Nucleolus organizer region
- Prognosis
ASJC Scopus subject areas
- Anatomy
- Histology
- Cell Biology
Cite this
Standardized AgNOR analysis as a prognostic parameter in endometrial carcinoma, endometrioid type. / Giuffrè, G.; Fulcheri, E.; Gualco, M.; Fedele, F.; Tuccari, G.
In: Analytical and Quantitative Cytology and Histology, Vol. 23, No. 1, 2001, p. 31-40.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Standardized AgNOR analysis as a prognostic parameter in endometrial carcinoma, endometrioid type
AU - Giuffrè, G.
AU - Fulcheri, E.
AU - Gualco, M.
AU - Fedele, F.
AU - Tuccari, G.
PY - 2001
Y1 - 2001
N2 - OBJECTIVE: To evaluate interobserver reproducibility of histologic grade in endometrial adenocarcinomas of endometrioid type (EC), to assess the relationships between nuclear grade and the amount of argyrophilic nucleolar organizer region (AgNOR) proteins and to determine the prognostic value of AgNOR proteins and the main clinicopathologic parameters. STUDY DESIGN: Architectural and nuclear grading were independently assessed by two pathologists in 64 formalin-fixed, paraffin-embedded surgical samples of EC obtained from an equal number of patients (age range, 38-84 years; mean, 63.5). Interobserver agreement was determined using the κ statistic; discrepant cases were reviewed, and a consensus was reached. Standardized AgNOR analysis was performed according to the guidelines of the Committee on AgNOR Quantification, measuring the mean area of AgNORs per nucleus (NORA) by an image analysis system. RESULTS: The κ values for interobserver agreement were substantial for architectural grading and moderate for nuclear grading. When NORA values were compared to the nuclear grade assessed by different observers, the most significant linear correlation (r = .713, P 4.212 μm2), while, by Cox multivariate analysis, AgNOR quantity emerged as an independent prognostic variable. CONCLUSION: Use of standardized AgNOR analysis may be an additional and objective tool in the assessment of histologic grade as well as a reliable method of determining prognosis in EC.
AB - OBJECTIVE: To evaluate interobserver reproducibility of histologic grade in endometrial adenocarcinomas of endometrioid type (EC), to assess the relationships between nuclear grade and the amount of argyrophilic nucleolar organizer region (AgNOR) proteins and to determine the prognostic value of AgNOR proteins and the main clinicopathologic parameters. STUDY DESIGN: Architectural and nuclear grading were independently assessed by two pathologists in 64 formalin-fixed, paraffin-embedded surgical samples of EC obtained from an equal number of patients (age range, 38-84 years; mean, 63.5). Interobserver agreement was determined using the κ statistic; discrepant cases were reviewed, and a consensus was reached. Standardized AgNOR analysis was performed according to the guidelines of the Committee on AgNOR Quantification, measuring the mean area of AgNORs per nucleus (NORA) by an image analysis system. RESULTS: The κ values for interobserver agreement were substantial for architectural grading and moderate for nuclear grading. When NORA values were compared to the nuclear grade assessed by different observers, the most significant linear correlation (r = .713, P 4.212 μm2), while, by Cox multivariate analysis, AgNOR quantity emerged as an independent prognostic variable. CONCLUSION: Use of standardized AgNOR analysis may be an additional and objective tool in the assessment of histologic grade as well as a reliable method of determining prognosis in EC.
KW - Endometrial neoplasms
KW - Nucleolus organizer region
KW - Prognosis
UR - http://www.scopus.com/inward/record.url?scp=0035115420&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035115420&partnerID=8YFLogxK
M3 - Article
C2 - 11233741
AN - SCOPUS:0035115420
VL - 23
SP - 31
EP - 40
JO - Analytical and Quantitative Cytology
JF - Analytical and Quantitative Cytology
SN - 0884-6812
IS - 1
ER -