Staphylococcus aureus impacts Pseudomonas aeruginosa chronic respiratory disease in murine models

C Cigana, I Bianconi, R Baldan, M De Simone, C Riva, B Sipione, G Rossi, DM Cirillo, A Bragonzi

Research output: Contribution to journalArticle

Abstract

Background: Staphylococcus aureus and Pseudomonas aeruginosa are key bacterial pathogens of the respiratory tract in patients with cystic fibrosis (CF). While P. aeruginosa chronic bronchial infection is associated with a poorer prognosis, the consequences of S. aureus colonization on CF outcomes are controversial. Methods: In this paper, murine models of infection resembling traits of the CF human airways disease have been revisited using an infection schedule that mimics the sequence of events of pulmonary disease in CF patients. First, mice were infected with S. aureus, embedded in agar beads; this was followed by P. aeruginosa infection and analysis of bacterial load, leukocyte infiltration, and lung tissue damage. Results: We reveal that: i) S. aureus promotes severe lesions including abscess formation, ii) S. aureus increases the risk of subsequent chronic P. aeruginosa respiratory infection, iii) once the chronic infection has been established, P. aeruginosa influences most of the inflammatory responses independent of S. aureus. Conclusions: Our findings established the significance of S. aureus colonization per se and the impact on the subsequent P. aeruginosa infection. This would point towards a thorough assessment for the need of treatment against S. aureus.
Original languageEnglish
Pages (from-to)933-942
Number of pages10
JournalThe Journal of infectious diseases
Volume217
Issue number6
DOIs
Publication statusPublished - 2018

Fingerprint

Pseudomonas aeruginosa
Staphylococcus aureus
Chronic Disease
Cystic Fibrosis
Pseudomonas Infections
Infection
Needs Assessment
Bacterial Load
Respiratory Tract Infections
Respiratory System
Abscess
Lung Diseases
Agar
Appointments and Schedules
Leukocytes
Lung

Cite this

Staphylococcus aureus impacts Pseudomonas aeruginosa chronic respiratory disease in murine models. / Cigana, C; Bianconi, I; Baldan, R; De Simone, M; Riva, C; Sipione, B; Rossi, G; Cirillo, DM; Bragonzi, A.

In: The Journal of infectious diseases, Vol. 217, No. 6, 2018, p. 933-942.

Research output: Contribution to journalArticle

@article{c545fd10283748da8a58e6b57598b51f,
title = "Staphylococcus aureus impacts Pseudomonas aeruginosa chronic respiratory disease in murine models",
abstract = "Background: Staphylococcus aureus and Pseudomonas aeruginosa are key bacterial pathogens of the respiratory tract in patients with cystic fibrosis (CF). While P. aeruginosa chronic bronchial infection is associated with a poorer prognosis, the consequences of S. aureus colonization on CF outcomes are controversial. Methods: In this paper, murine models of infection resembling traits of the CF human airways disease have been revisited using an infection schedule that mimics the sequence of events of pulmonary disease in CF patients. First, mice were infected with S. aureus, embedded in agar beads; this was followed by P. aeruginosa infection and analysis of bacterial load, leukocyte infiltration, and lung tissue damage. Results: We reveal that: i) S. aureus promotes severe lesions including abscess formation, ii) S. aureus increases the risk of subsequent chronic P. aeruginosa respiratory infection, iii) once the chronic infection has been established, P. aeruginosa influences most of the inflammatory responses independent of S. aureus. Conclusions: Our findings established the significance of S. aureus colonization per se and the impact on the subsequent P. aeruginosa infection. This would point towards a thorough assessment for the need of treatment against S. aureus.",
author = "C Cigana and I Bianconi and R Baldan and {De Simone}, M and C Riva and B Sipione and G Rossi and DM Cirillo and A Bragonzi",
year = "2018",
doi = "10.1093/infdis/jix621",
language = "English",
volume = "217",
pages = "933--942",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "6",

}

TY - JOUR

T1 - Staphylococcus aureus impacts Pseudomonas aeruginosa chronic respiratory disease in murine models

AU - Cigana, C

AU - Bianconi, I

AU - Baldan, R

AU - De Simone, M

AU - Riva, C

AU - Sipione, B

AU - Rossi, G

AU - Cirillo, DM

AU - Bragonzi, A

PY - 2018

Y1 - 2018

N2 - Background: Staphylococcus aureus and Pseudomonas aeruginosa are key bacterial pathogens of the respiratory tract in patients with cystic fibrosis (CF). While P. aeruginosa chronic bronchial infection is associated with a poorer prognosis, the consequences of S. aureus colonization on CF outcomes are controversial. Methods: In this paper, murine models of infection resembling traits of the CF human airways disease have been revisited using an infection schedule that mimics the sequence of events of pulmonary disease in CF patients. First, mice were infected with S. aureus, embedded in agar beads; this was followed by P. aeruginosa infection and analysis of bacterial load, leukocyte infiltration, and lung tissue damage. Results: We reveal that: i) S. aureus promotes severe lesions including abscess formation, ii) S. aureus increases the risk of subsequent chronic P. aeruginosa respiratory infection, iii) once the chronic infection has been established, P. aeruginosa influences most of the inflammatory responses independent of S. aureus. Conclusions: Our findings established the significance of S. aureus colonization per se and the impact on the subsequent P. aeruginosa infection. This would point towards a thorough assessment for the need of treatment against S. aureus.

AB - Background: Staphylococcus aureus and Pseudomonas aeruginosa are key bacterial pathogens of the respiratory tract in patients with cystic fibrosis (CF). While P. aeruginosa chronic bronchial infection is associated with a poorer prognosis, the consequences of S. aureus colonization on CF outcomes are controversial. Methods: In this paper, murine models of infection resembling traits of the CF human airways disease have been revisited using an infection schedule that mimics the sequence of events of pulmonary disease in CF patients. First, mice were infected with S. aureus, embedded in agar beads; this was followed by P. aeruginosa infection and analysis of bacterial load, leukocyte infiltration, and lung tissue damage. Results: We reveal that: i) S. aureus promotes severe lesions including abscess formation, ii) S. aureus increases the risk of subsequent chronic P. aeruginosa respiratory infection, iii) once the chronic infection has been established, P. aeruginosa influences most of the inflammatory responses independent of S. aureus. Conclusions: Our findings established the significance of S. aureus colonization per se and the impact on the subsequent P. aeruginosa infection. This would point towards a thorough assessment for the need of treatment against S. aureus.

U2 - 10.1093/infdis/jix621

DO - 10.1093/infdis/jix621

M3 - Article

VL - 217

SP - 933

EP - 942

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 6

ER -