Stem cell factor activates telomerase in mouse mitotic spermatogonia and in primordial germ cells

Sussanna Dolci, Lauretta Levati, Manuela Pellegrini, Isabella Faraoni, Grazia Graziani, Anna Di Carlo, Raffaele Geremia

Research output: Contribution to journalArticle

Abstract

The discovery of sterility in the descendants of telomerase-null mutant mice, owing to the lack of spermatogonia proliferation, has drawn attention to the role of telomerase activity in mouse spermatogenesis. Since spermatogonia proliferation is under Kitl control, we explored its possible role in the regulation of telomerase activity. We show that Kitl induces telomerase activity in mitotic spermatogonia and increases the mRNA levels of both the catalytic subunit form and the telomerase RNA template. The increase of telomerase activity by Kitl is blocked by the presence of the PI3K inhibitor LY294002. Kit-positive proliferating male primordial germ cells (PGCs) show low levels of telomerase activity, but they increase telomerase activity upon Kitl stimulation. Diplotene-arrested growing oocytes that reexpress Kit do not increase telomerase activity upon Kitl stimulation. Our data suggest that the induction of telomerase by Kitl may contribute to the self-renewing potential of male germ cells and of PGCs.

Original languageEnglish
Pages (from-to)1643-1649
Number of pages7
JournalJournal of Cell Science
Volume115
Issue number8
Publication statusPublished - May 15 2002

Keywords

  • Germ cells
  • Kit
  • Kitl
  • Meiosis
  • PI3K
  • Proliferation
  • Telomerase

ASJC Scopus subject areas

  • Cell Biology

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    Dolci, S., Levati, L., Pellegrini, M., Faraoni, I., Graziani, G., Di Carlo, A., & Geremia, R. (2002). Stem cell factor activates telomerase in mouse mitotic spermatogonia and in primordial germ cells. Journal of Cell Science, 115(8), 1643-1649.