Stem cell-mediated muscle regeneration is enhanced by local isoform of insulin-like growth factor 1

Antonio Musaró, Cristina Giacinti, Giovanna Borsellino, Gabriella Dobrowolny, Laura Pelosi, Linda Cairns, Sergio Ottolenghi, Giulio Cossu, Giorgio Bernardi, Luca Battistini, Mario Molinaro, Nadia Rosenthal

Research output: Contribution to journalArticle

Abstract

We investigated the mechanism whereby expression of a transgene encoding a locally acting isoform of insulin-like growth factor 1 (mlGF-1) enhances repair of skeletal muscle damage. Increased recruitment of proliferating bone marrow cells to injured MLC/mlgf-1 transgenic muscles was accompanied by elevated bone marrow stem cell production in response to distal trauma. Regenerating MLC/mlgf-1 transgenic muscles contained increased cell populations expressing stem cell markers, exhibited accelerated myogenic differentiation, expressed markers of regeneration and readily converted cocultured bone marrow to muscle. These data implicate mlGF-1 as a powerful enhancer of the regeneration response, mediating the recruitment of bone marrow cells to sites of tissue damage and augmenting local repair mechanisms.

Original languageEnglish
Pages (from-to)1206-1210
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number5
DOIs
Publication statusPublished - Feb 3 2004

    Fingerprint

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Musaró, A., Giacinti, C., Borsellino, G., Dobrowolny, G., Pelosi, L., Cairns, L., Ottolenghi, S., Cossu, G., Bernardi, G., Battistini, L., Molinaro, M., & Rosenthal, N. (2004). Stem cell-mediated muscle regeneration is enhanced by local isoform of insulin-like growth factor 1. Proceedings of the National Academy of Sciences of the United States of America, 101(5), 1206-1210. https://doi.org/10.1073/pnas.0303792101