Stem Cell Modeling of Neuroferritinopathy Reveals Iron as a Determinant of Senescence and Ferroptosis during Neuronal Aging

Anna Cozzi, Daniel I Orellana, Paolo Santambrogio, Alicia Rubio, Cinzia Cancellieri, Serena Giannelli, Maddalena Ripamonti, Stefano Taverna, Giulia Di Lullo, Ermanna Rovida, Maurizio Ferrari, Gian Luca Forni, Chiara Fiorillo, Vania Broccoli, Sonia Levi

Research output: Contribution to journalArticlepeer-review

Abstract

Neuroferritinopathy (NF) is a movement disorder caused by alterations in the L-ferritin gene that generate cytosolic free iron. NF is a unique pathophysiological model for determining the direct consequences of cell iron dysregulation. We established lines of induced pluripotent stem cells from fibroblasts from two NF patients and one isogenic control obtained by CRISPR/Cas9 technology. NF fibroblasts, neural progenitors, and neurons exhibited the presence of increased cytosolic iron, which was also detectable as: ferritin aggregates, alterations in the iron parameters, oxidative damage, and the onset of a senescence phenotype, particularly severe in the neurons. In this spontaneous senescence model, NF cells had impaired survival and died by ferroptosis. Thus, non-ferritin-bound iron is sufficient per se to cause both cell senescence and ferroptotic cell death in human fibroblasts and neurons. These results provide strong evidence supporting the primary role of iron in neuronal aging and degeneration.

Original languageEnglish
Pages (from-to)832-846
Number of pages15
JournalStem Cell Reports
Volume13
Issue number5
DOIs
Publication statusPublished - Nov 12 2019

Fingerprint Dive into the research topics of 'Stem Cell Modeling of Neuroferritinopathy Reveals Iron as a Determinant of Senescence and Ferroptosis during Neuronal Aging'. Together they form a unique fingerprint.

Cite this