Abstract
Cutaneous melanoma is an extremely heterogenous human cancer. The most aggressive melanoma may contain deregulated cells with undifferentiated/stem cell-like phenotype. A critical mechanism by which melanoma cells enhance their invasive capacity is the dissolution of the intercellular adhesion and the acquisition of mesenchymal features as a part of an epithelial-to-mesenchymal transition. The aim of this study was to clarify the role of a stem cell-like population in human melanomas by means of melanocytic cell culture analysis obtained from distinct histotypes of primary and metastatic malignant melanoma. Patients with advanced melanoma >2cm in diameter and/or >300mm2 surface were enrolled. The melanoma cells were isolated from skin biopsies of lentigo maligna melanoma, superficial spreading melanoma, nodular melanoma, and metastatic melanoma. The colony forming unit assay and alkaline phosphatase stain were evaluated. Cells were subsequently cultured and maintained in different media to evaluate their ability to differentiate into osteogenic and adipogenic lineages. Immunohistochemistry and flow cytometry analysis were performed to evaluate antigenic markers CD90, CD73, CD105, CD146, CD20, CD166, and Nestin. This study confirms that melanoma can include heterogenous cell populations with the ability both to self-renew and to a give rise to differentiated progeny. Melanoma cells displayed intratumoral heterogeneity and dynamic antigen phenotypes. Histologically, transitions from normal skin to melanoma were associated with a gradual increase in the expression of CD146, CD20, CD133, Nestin, and CD73. These molecular profiles could be further analyzed and, in the future, used for the development of novel biomolecular targeted-therapy approaches.
| Original language | English |
|---|---|
| Pages (from-to) | 171-181 |
| Number of pages | 11 |
| Journal | Applied Immunohistochemistry and Molecular Morphology |
| Volume | 22 |
| Issue number | 3 |
| Publication status | Published - 2014 |
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Keywords
- CD133
- CD166
- CD20
- Malignant melanoma
- Melanoma cell cultures
- Mesenchymal stem cell antigens
- Nestin
- S100
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Medical Laboratory Technology
- Histology
- Medicine(all)
Cite this
Stem cell properties in cell cultures from different stage of melanoma progression. / Magnoni, Cristina; Giudice, Stefania; Pellacani, Giovanni; Bertazzoni, Giorgia; Longo, Caterina; Veratti, Eugenia; Morini, Daria; Benassi, Luisa; Vaschieri, Cristina; Azzoni, Paola; De Pol, Anto; Seidenari, Stefania; Tomasi, Aldo; Pollio, Annamaria; Ponti, Giovanniv.
In: Applied Immunohistochemistry and Molecular Morphology, Vol. 22, No. 3, 2014, p. 171-181.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stem cell properties in cell cultures from different stage of melanoma progression
AU - Magnoni, Cristina
AU - Giudice, Stefania
AU - Pellacani, Giovanni
AU - Bertazzoni, Giorgia
AU - Longo, Caterina
AU - Veratti, Eugenia
AU - Morini, Daria
AU - Benassi, Luisa
AU - Vaschieri, Cristina
AU - Azzoni, Paola
AU - De Pol, Anto
AU - Seidenari, Stefania
AU - Tomasi, Aldo
AU - Pollio, Annamaria
AU - Ponti, Giovanniv
PY - 2014
Y1 - 2014
N2 - Cutaneous melanoma is an extremely heterogenous human cancer. The most aggressive melanoma may contain deregulated cells with undifferentiated/stem cell-like phenotype. A critical mechanism by which melanoma cells enhance their invasive capacity is the dissolution of the intercellular adhesion and the acquisition of mesenchymal features as a part of an epithelial-to-mesenchymal transition. The aim of this study was to clarify the role of a stem cell-like population in human melanomas by means of melanocytic cell culture analysis obtained from distinct histotypes of primary and metastatic malignant melanoma. Patients with advanced melanoma >2cm in diameter and/or >300mm2 surface were enrolled. The melanoma cells were isolated from skin biopsies of lentigo maligna melanoma, superficial spreading melanoma, nodular melanoma, and metastatic melanoma. The colony forming unit assay and alkaline phosphatase stain were evaluated. Cells were subsequently cultured and maintained in different media to evaluate their ability to differentiate into osteogenic and adipogenic lineages. Immunohistochemistry and flow cytometry analysis were performed to evaluate antigenic markers CD90, CD73, CD105, CD146, CD20, CD166, and Nestin. This study confirms that melanoma can include heterogenous cell populations with the ability both to self-renew and to a give rise to differentiated progeny. Melanoma cells displayed intratumoral heterogeneity and dynamic antigen phenotypes. Histologically, transitions from normal skin to melanoma were associated with a gradual increase in the expression of CD146, CD20, CD133, Nestin, and CD73. These molecular profiles could be further analyzed and, in the future, used for the development of novel biomolecular targeted-therapy approaches.
AB - Cutaneous melanoma is an extremely heterogenous human cancer. The most aggressive melanoma may contain deregulated cells with undifferentiated/stem cell-like phenotype. A critical mechanism by which melanoma cells enhance their invasive capacity is the dissolution of the intercellular adhesion and the acquisition of mesenchymal features as a part of an epithelial-to-mesenchymal transition. The aim of this study was to clarify the role of a stem cell-like population in human melanomas by means of melanocytic cell culture analysis obtained from distinct histotypes of primary and metastatic malignant melanoma. Patients with advanced melanoma >2cm in diameter and/or >300mm2 surface were enrolled. The melanoma cells were isolated from skin biopsies of lentigo maligna melanoma, superficial spreading melanoma, nodular melanoma, and metastatic melanoma. The colony forming unit assay and alkaline phosphatase stain were evaluated. Cells were subsequently cultured and maintained in different media to evaluate their ability to differentiate into osteogenic and adipogenic lineages. Immunohistochemistry and flow cytometry analysis were performed to evaluate antigenic markers CD90, CD73, CD105, CD146, CD20, CD166, and Nestin. This study confirms that melanoma can include heterogenous cell populations with the ability both to self-renew and to a give rise to differentiated progeny. Melanoma cells displayed intratumoral heterogeneity and dynamic antigen phenotypes. Histologically, transitions from normal skin to melanoma were associated with a gradual increase in the expression of CD146, CD20, CD133, Nestin, and CD73. These molecular profiles could be further analyzed and, in the future, used for the development of novel biomolecular targeted-therapy approaches.
KW - CD133
KW - CD166
KW - CD20
KW - Malignant melanoma
KW - Melanoma cell cultures
KW - Mesenchymal stem cell antigens
KW - Nestin
KW - S100
UR - http://www.scopus.com/inward/record.url?scp=84910128795&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84910128795&partnerID=8YFLogxK
M3 - Article
C2 - 23702651
AN - SCOPUS:84910128795
VL - 22
SP - 171
EP - 181
JO - Applied Immunohistochemistry and Molecular Morphology
JF - Applied Immunohistochemistry and Molecular Morphology
SN - 1541-2016
IS - 3
ER -