Sterol and steroid catabolites from cholesterol produced by the psychrophile Pseudoalteromonas haloplanktis

Monica Gelzo, Anna Lamberti, Giuseppe Spano, Antonio Dello Russo, Gaetano Corso, Mariorosario Masullo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pseudoalteromonas haloplanktis, a psychrotrophilic marine bacterium of biotechnological interest, shows anti-biofilm properties and is particularly relevant for cold storage of vacuum packed seafood. We focused our interest on the activation of cholesterol metabolism in this bacterium as the presence in its genome of a putative 3-ketosteroid-Δ1-dehydrogenase. This study reports GC-MS and LC-MS/MS profiles of sterols/steroids and their derivatives found in cell extracts of P. haloplanktis grown in a medium with a low content of cholesterol. Here, for the first time, we suggest that P. haloplanktis produces some intermediates of cholesterol catabolism, putatively identified as 24-hydroxycholest-1,4-dien-3-one-26-oic acid, chol-1,4-dien-3-one-24-oic acid, 26-hydroxycholest-4-en-3-one, and pregn-4-en-3-one-20-carboxylic acid, a finding already reported in other microorganisms. The presence of these compounds, also considered steroid precursors, produced by P. haloplanktis in vacuum packed seafood could be of interest for healthy of consumers, as well as, for biotechnological applications in pharmaceutical industry.

Original languageEnglish
Pages (from-to)947-951
Number of pages5
JournalJournal of Mass Spectrometry
Volume49
Issue number9
DOIs
Publication statusPublished - Sep 1 2014

Fingerprint

psychrophiles
Pseudoalteromonas
steroids
Cholesterol
cholesterol
Sterols
Seafood
Steroids
Vacuum
bacteria
Bacteria
catabolism
Ketosteroids
Cold storage
vacuum
biofilms
acids
Acids
dehydrogenases
genome

Keywords

  • Cholesterol catabolism
  • GC-MS
  • LC-MS/MS
  • Pseudoalteromonas haloplanktis
  • Steroids

ASJC Scopus subject areas

  • Spectroscopy
  • Medicine(all)

Cite this

Sterol and steroid catabolites from cholesterol produced by the psychrophile Pseudoalteromonas haloplanktis. / Gelzo, Monica; Lamberti, Anna; Spano, Giuseppe; Dello Russo, Antonio; Corso, Gaetano; Masullo, Mariorosario.

In: Journal of Mass Spectrometry, Vol. 49, No. 9, 01.09.2014, p. 947-951.

Research output: Contribution to journalArticle

Gelzo, Monica ; Lamberti, Anna ; Spano, Giuseppe ; Dello Russo, Antonio ; Corso, Gaetano ; Masullo, Mariorosario. / Sterol and steroid catabolites from cholesterol produced by the psychrophile Pseudoalteromonas haloplanktis. In: Journal of Mass Spectrometry. 2014 ; Vol. 49, No. 9. pp. 947-951.
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