Stimulation of group I mGlu receptors in the ventrotegmental area enhances extracellular dopamine in the rat medial prefrontal cortex

G. Renoldi, E. Calcagno, F. Borsini, R. W. Invernizzi

Research output: Contribution to journalArticlepeer-review

Abstract

Group I mGlu receptors have been implicated in the control of brain dopamine release. However, the receptor subtype involved and the precise site of action have not been determined. In this study we show that (R,S)3,5-dihydroxyphenylglycine (DHPG; 6 and 60 nmol ICV), a selective group I mGlu receptor agonist, raised extracellular dopamine respectively by 176% and 243% of basal values in the medial prefrontal cortex as assessed by in vivo microdialysis in conscious rats. (R,S)2-chloro-5-hydroxyphenylglycine (60 nmol ICV), a selective mGlu5 receptor agonist, raised extracellular dopamine by 396% of basal values. Intra-VTA DHPG (0.6-6 nmol) mimicked ICV injection whereas intracortical infusion (1-1000 μmol/L) had no effect. DHPG-induced rise of extracellular dopamine was reversed by tetrodotoxin and by the selective mGlu1 and mGlu5 receptor antagonists 7(hydroxyimino)cyclopropa[b]chromen-1a- carboxylate (CPCCOEt) and 2-methyl-6-(phenylethynyl)pyridine (MPEP) either ICV or into the ventrotegmental area (VTA), suggesting that neuronal release and both mGlu1 and mGlu5 receptors were involved. These results support the existence of functional mGlu1 and mGlu5 receptors in the VTA regulating the release of dopamine in the medial prefrontal cortex.

Original languageEnglish
Pages (from-to)1658-1666
Number of pages9
JournalJournal of Neurochemistry
Volume100
Issue number6
DOIs
Publication statusPublished - Mar 2007

Keywords

  • (R,S)3,5-dihydroxyphenylglycine
  • 2-methyl-6-(phenylethynyl)pyridine
  • 7(hydroxyimino)cyclopropa[b]chromen-1a- carboxylate
  • Dopamine
  • Medial prefrontal cortex
  • mGlu1 and mGlu5 receptors
  • Ventrotegmental area

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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