Abstract
The biomolecular mechanisms that mediate signal transduction by type 11 (γ) interferon (IFN) are poorly understood. IFN-γ is a potent growth inhibitory cytokine also endowed with antiviral, immunomodulatory, and differentiating activities on various cell targets, including neural cells. IFN-γ induced a rapid and transient activation of phospholipase A2 in LAN-5, a human neuroblastoma cell line. A consequence of phospholipase A2 activation was the release of arachidonic acid and the generation of lysophospholipids from membrane phospholipids. Treatment of pre-labeled LAN-5 cells with a receptor-saturating concentration of IFN-γ led to a time-dependent release of [3H]arachidonic acid into the culture media and generation or [32P]lysophosphatidylcholine. Pretreatment of cultures with the phospholipase A2 inhibitor, bromophenacyl bromide, markedly inhibited both [3H]arachidonic acid release and lysophosphatidylcholine production induced by IFN-γ treatment. Pretreatment of LAN-5 cells with nordihydroguaiaretic acid, a lipoxygenase inhibitor, or with indomethacin, a cyclooxygenase inhibitor, amplified the release of[3H]arachidonic acid and production of lysophosphatidylcholine induced by non-saturating concentrations of IFN-γ. In parallel, and with the same time-dependent effect, a significant decrease in phosphatidylcholine labeling was observed in IFN-γ-treated cells, further indicating that a potential signal transduction mechanism of IFN-γ is the hydrolysis of membrane phosphatidylcholine by phospholipase A2.
Original language | English |
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Pages (from-to) | 17-21 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 310 |
Issue number | 1 |
DOIs | |
Publication status | Published - Sep 21 1992 |
Keywords
- Indomethacin
- Interferon-γ
- Neuroblastoma
- Nordihydroguaiaretic acid (NDGA)
- Phospholipase A
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology