Stimulation of receptor-coupled phospholipase A2 by interferon-γ

Mirco Ponzoni, Paolo G. Montaldo, Paolo Cornaglia-Ferraris

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The biomolecular mechanisms that mediate signal transduction by type 11 (γ) interferon (IFN) are poorly understood. IFN-γ is a potent growth inhibitory cytokine also endowed with antiviral, immunomodulatory, and differentiating activities on various cell targets, including neural cells. IFN-γ induced a rapid and transient activation of phospholipase A2 in LAN-5, a human neuroblastoma cell line. A consequence of phospholipase A2 activation was the release of arachidonic acid and the generation of lysophospholipids from membrane phospholipids. Treatment of pre-labeled LAN-5 cells with a receptor-saturating concentration of IFN-γ led to a time-dependent release of [3H]arachidonic acid into the culture media and generation or [32P]lysophosphatidylcholine. Pretreatment of cultures with the phospholipase A2 inhibitor, bromophenacyl bromide, markedly inhibited both [3H]arachidonic acid release and lysophosphatidylcholine production induced by IFN-γ treatment. Pretreatment of LAN-5 cells with nordihydroguaiaretic acid, a lipoxygenase inhibitor, or with indomethacin, a cyclooxygenase inhibitor, amplified the release of[3H]arachidonic acid and production of lysophosphatidylcholine induced by non-saturating concentrations of IFN-γ. In parallel, and with the same time-dependent effect, a significant decrease in phosphatidylcholine labeling was observed in IFN-γ-treated cells, further indicating that a potential signal transduction mechanism of IFN-γ is the hydrolysis of membrane phosphatidylcholine by phospholipase A2.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalFEBS Letters
Volume310
Issue number1
DOIs
Publication statusPublished - Sep 21 1992

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Phospholipase A2 Receptors
Phospholipases A2
Interferons
Local Area Networks
Arachidonic Acid
Lysophosphatidylcholines
Local area networks
Signal transduction
Phosphatidylcholines
Signal Transduction
Chemical activation
Cells
Masoprocol
Lysophospholipids
Membranes
Lipoxygenase Inhibitors
Cyclooxygenase Inhibitors
Neuroblastoma
Indomethacin
Labeling

Keywords

  • Indomethacin
  • Interferon-γ
  • Neuroblastoma
  • Nordihydroguaiaretic acid (NDGA)
  • Phospholipase A

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Stimulation of receptor-coupled phospholipase A2 by interferon-γ. / Ponzoni, Mirco; Montaldo, Paolo G.; Cornaglia-Ferraris, Paolo.

In: FEBS Letters, Vol. 310, No. 1, 21.09.1992, p. 17-21.

Research output: Contribution to journalArticle

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