Stimulation of receptor-coupled phospholipase A2 by interferon-γ

Mirco Ponzoni, Paolo G. Montaldo, Paolo Cornaglia-Ferraris

Research output: Contribution to journalArticlepeer-review

Abstract

The biomolecular mechanisms that mediate signal transduction by type 11 (γ) interferon (IFN) are poorly understood. IFN-γ is a potent growth inhibitory cytokine also endowed with antiviral, immunomodulatory, and differentiating activities on various cell targets, including neural cells. IFN-γ induced a rapid and transient activation of phospholipase A2 in LAN-5, a human neuroblastoma cell line. A consequence of phospholipase A2 activation was the release of arachidonic acid and the generation of lysophospholipids from membrane phospholipids. Treatment of pre-labeled LAN-5 cells with a receptor-saturating concentration of IFN-γ led to a time-dependent release of [3H]arachidonic acid into the culture media and generation or [32P]lysophosphatidylcholine. Pretreatment of cultures with the phospholipase A2 inhibitor, bromophenacyl bromide, markedly inhibited both [3H]arachidonic acid release and lysophosphatidylcholine production induced by IFN-γ treatment. Pretreatment of LAN-5 cells with nordihydroguaiaretic acid, a lipoxygenase inhibitor, or with indomethacin, a cyclooxygenase inhibitor, amplified the release of[3H]arachidonic acid and production of lysophosphatidylcholine induced by non-saturating concentrations of IFN-γ. In parallel, and with the same time-dependent effect, a significant decrease in phosphatidylcholine labeling was observed in IFN-γ-treated cells, further indicating that a potential signal transduction mechanism of IFN-γ is the hydrolysis of membrane phosphatidylcholine by phospholipase A2.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalFEBS Letters
Volume310
Issue number1
DOIs
Publication statusPublished - Sep 21 1992

Keywords

  • Indomethacin
  • Interferon-γ
  • Neuroblastoma
  • Nordihydroguaiaretic acid (NDGA)
  • Phospholipase A

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Stimulation of receptor-coupled phospholipase A2 by interferon-γ'. Together they form a unique fingerprint.

Cite this