Storage pool disease: Comparative fluorescence microscopical, cytochemical and biochemical studies on amine-storing organelles of human blood platelets

H. P. Lorez, J. G. Richards, M. da Prada, G. B. Picotti, F. I. Pareti, A. Capitanio, P. M. Mannucci

Research output: Contribution to journalArticle

Abstract

The platelet content of ATP, ADP, serotonin (5-HT), dopamine, noradrenaline and adrenaline as well as the net uptake of radiolabelled 5-HT and mepacrine were subnormal in six patients with Storage Pool Disease (SPD). Fewer amine-storing organelles were found by fluorescence microscopy with the fluorescent probe mepacrine and by electron microscopy with a cytochemical (uranaffin) reaction specific for 5'-phosphonucleotides. Both methods showed that SPD platelets have atypical organelles with a reduced capacity to store amines, 5'-phosphonucleotides and mepacrine. The changes were most marked in platelets of the two patients who also had oculocutaneous albinism.

Original languageEnglish
Pages (from-to)297-305
Number of pages9
JournalBritish Journal of Haematology
Volume43
Issue number2
Publication statusPublished - 1979

ASJC Scopus subject areas

  • Hematology

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