Straightforward relative quantitation and age-related human standards of N-acetylaspartate at the centrum semiovale level by CSI 1H-MRS

E. Rotondo, G. Bruschetta, A. Saccà, P. Bramanti, M. R. Di Pasquale

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

1H-MRS was aimed to monitor metabolite concentrations in homogeneous interaxial slices of cerebral matter at the centrum semiovale level in healthy volunteers. NAA (N-acetylaspartate + N-acetylglutamate), Cr (creatine + phosphocreatine), and Cho (choline + acetylcholine) were evaluated by resonance integrations. Using Cr as an internal standard, NAA/Cr ratio was considered as a relative measure of concentration. CSI sequence explored volunteer's interaxial slices of white and gray matter by means of 8 × 8 matrices of 1H-NMR spectra. NAA/Cr integral ratios were averaged over the whole spectral matrix to obtain the Index of NAA at Centrum Semiovale (INACS) of each individual. Indexes of the sixty-eight healthy volunteers, divided into three groups by age, showed good intraindividual reproducibility, and were virtually unaffected by small shifts or bendings of the interaxial slice analyzed. The INACSs were used to estimate Age-Sectorial INACS Ranges (ASIR), the intervals that, on the basis of a normal statistical distribution, should comprise 95% of the age-matched healthy population. Individual INACSs, compared to accurately defined ASIRs taken as standards, could early detect subtle, diffuse neuronal or axonal damage within centrum semiovale interaxial slices. Periodic inspection of INACS could also allow monitoring of progressive neuronal or axonal degeneration.

Original languageEnglish
Pages (from-to)1055-1060
Number of pages6
JournalMagnetic Resonance Imaging
Volume21
Issue number9
DOIs
Publication statusPublished - Nov 2003

Fingerprint

Metabolites
Healthy Volunteers
Inspection
Nuclear magnetic resonance
creatine
Statistical Distributions
acetylcholine
choline
Phosphocreatine
degeneration
Creatine
Monitoring
metabolites
Normal Distribution
matrices
Choline
statistical distributions
Acetylcholine
inspection
Volunteers

Keywords

  • H-MRS
  • Age-sectorial INACS range (ASIR)
  • Chemical shift imaging (CSI)
  • Index of NAA at centrum semiovale (INACS)

ASJC Scopus subject areas

  • Biophysics
  • Clinical Biochemistry
  • Structural Biology
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

Straightforward relative quantitation and age-related human standards of N-acetylaspartate at the centrum semiovale level by CSI 1H-MRS. / Rotondo, E.; Bruschetta, G.; Saccà, A.; Bramanti, P.; Di Pasquale, M. R.

In: Magnetic Resonance Imaging, Vol. 21, No. 9, 11.2003, p. 1055-1060.

Research output: Contribution to journalArticle

@article{21d401f2f715496387cd898ec2edc6d9,
title = "Straightforward relative quantitation and age-related human standards of N-acetylaspartate at the centrum semiovale level by CSI 1H-MRS",
abstract = "1H-MRS was aimed to monitor metabolite concentrations in homogeneous interaxial slices of cerebral matter at the centrum semiovale level in healthy volunteers. NAA (N-acetylaspartate + N-acetylglutamate), Cr (creatine + phosphocreatine), and Cho (choline + acetylcholine) were evaluated by resonance integrations. Using Cr as an internal standard, NAA/Cr ratio was considered as a relative measure of concentration. CSI sequence explored volunteer's interaxial slices of white and gray matter by means of 8 × 8 matrices of 1H-NMR spectra. NAA/Cr integral ratios were averaged over the whole spectral matrix to obtain the Index of NAA at Centrum Semiovale (INACS) of each individual. Indexes of the sixty-eight healthy volunteers, divided into three groups by age, showed good intraindividual reproducibility, and were virtually unaffected by small shifts or bendings of the interaxial slice analyzed. The INACSs were used to estimate Age-Sectorial INACS Ranges (ASIR), the intervals that, on the basis of a normal statistical distribution, should comprise 95{\%} of the age-matched healthy population. Individual INACSs, compared to accurately defined ASIRs taken as standards, could early detect subtle, diffuse neuronal or axonal damage within centrum semiovale interaxial slices. Periodic inspection of INACS could also allow monitoring of progressive neuronal or axonal degeneration.",
keywords = "H-MRS, Age-sectorial INACS range (ASIR), Chemical shift imaging (CSI), Index of NAA at centrum semiovale (INACS)",
author = "E. Rotondo and G. Bruschetta and A. Sacc{\`a} and P. Bramanti and {Di Pasquale}, {M. R.}",
year = "2003",
month = "11",
doi = "10.1016/S0730-725X(03)00211-X",
language = "English",
volume = "21",
pages = "1055--1060",
journal = "Magnetic Resonance Imaging",
issn = "0730-725X",
publisher = "Elsevier Inc.",
number = "9",

}

TY - JOUR

T1 - Straightforward relative quantitation and age-related human standards of N-acetylaspartate at the centrum semiovale level by CSI 1H-MRS

AU - Rotondo, E.

AU - Bruschetta, G.

AU - Saccà, A.

AU - Bramanti, P.

AU - Di Pasquale, M. R.

PY - 2003/11

Y1 - 2003/11

N2 - 1H-MRS was aimed to monitor metabolite concentrations in homogeneous interaxial slices of cerebral matter at the centrum semiovale level in healthy volunteers. NAA (N-acetylaspartate + N-acetylglutamate), Cr (creatine + phosphocreatine), and Cho (choline + acetylcholine) were evaluated by resonance integrations. Using Cr as an internal standard, NAA/Cr ratio was considered as a relative measure of concentration. CSI sequence explored volunteer's interaxial slices of white and gray matter by means of 8 × 8 matrices of 1H-NMR spectra. NAA/Cr integral ratios were averaged over the whole spectral matrix to obtain the Index of NAA at Centrum Semiovale (INACS) of each individual. Indexes of the sixty-eight healthy volunteers, divided into three groups by age, showed good intraindividual reproducibility, and were virtually unaffected by small shifts or bendings of the interaxial slice analyzed. The INACSs were used to estimate Age-Sectorial INACS Ranges (ASIR), the intervals that, on the basis of a normal statistical distribution, should comprise 95% of the age-matched healthy population. Individual INACSs, compared to accurately defined ASIRs taken as standards, could early detect subtle, diffuse neuronal or axonal damage within centrum semiovale interaxial slices. Periodic inspection of INACS could also allow monitoring of progressive neuronal or axonal degeneration.

AB - 1H-MRS was aimed to monitor metabolite concentrations in homogeneous interaxial slices of cerebral matter at the centrum semiovale level in healthy volunteers. NAA (N-acetylaspartate + N-acetylglutamate), Cr (creatine + phosphocreatine), and Cho (choline + acetylcholine) were evaluated by resonance integrations. Using Cr as an internal standard, NAA/Cr ratio was considered as a relative measure of concentration. CSI sequence explored volunteer's interaxial slices of white and gray matter by means of 8 × 8 matrices of 1H-NMR spectra. NAA/Cr integral ratios were averaged over the whole spectral matrix to obtain the Index of NAA at Centrum Semiovale (INACS) of each individual. Indexes of the sixty-eight healthy volunteers, divided into three groups by age, showed good intraindividual reproducibility, and were virtually unaffected by small shifts or bendings of the interaxial slice analyzed. The INACSs were used to estimate Age-Sectorial INACS Ranges (ASIR), the intervals that, on the basis of a normal statistical distribution, should comprise 95% of the age-matched healthy population. Individual INACSs, compared to accurately defined ASIRs taken as standards, could early detect subtle, diffuse neuronal or axonal damage within centrum semiovale interaxial slices. Periodic inspection of INACS could also allow monitoring of progressive neuronal or axonal degeneration.

KW - H-MRS

KW - Age-sectorial INACS range (ASIR)

KW - Chemical shift imaging (CSI)

KW - Index of NAA at centrum semiovale (INACS)

UR - http://www.scopus.com/inward/record.url?scp=0347478285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347478285&partnerID=8YFLogxK

U2 - 10.1016/S0730-725X(03)00211-X

DO - 10.1016/S0730-725X(03)00211-X

M3 - Article

VL - 21

SP - 1055

EP - 1060

JO - Magnetic Resonance Imaging

JF - Magnetic Resonance Imaging

SN - 0730-725X

IS - 9

ER -