Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells

Maria I. Almeida, Milena S. Nicoloso, Lizhi Zeng, Cristina Ivan, Riccardo Spizzo, Roberta Gafà, Lianchun Xiao, Xinna Zhang, Ivan Vannini, Francesca Fanini, Muller Fabbri, Giovanni Lanza, Rui M. Reis, Patrick A. Zweidler-Mckay, George A. Calin

Research output: Contribution to journalArticlepeer-review

Abstract

Background & Aims: MicroRNAs (miRNAs) can promote or inhibit tumor growth and are therefore being developed as targets for cancer therapies. They are diverse not only in the messenger RNAs (mRNA) they target, but in their production; the same hairpin RNA structure can generate mature products from each strand, termed 5p and 3p, that can bind different mRNAs. We analyzed the expression, functions, and mechanisms of miR-28-5p and miR-28-3p in colorectal cancer (CRC) cells. Methods: We measured levels of miR-28-5p and miR-28-3p expression in 108 CRC and 49 normal colorectal samples (47 paired) by reverse transcription, quantitative real-time polymerase chain reaction. The roles of miR-28 in CRC development were studied using cultured HCT116, RKO, and SW480 cells and tumor xenograft analyses in immunodeficient mice; their mRNA targets were also investigated. Results: miR-28-5p and miR-28-3p were down-regulated in CRC samples compared with normal colon samples. Overexpression of miRNAs in CRC cells had different effects and the miRNAs interacted with different mRNAs: miR-28-5p altered expression of CCND1 and HOXB3, whereas miR-28-3p bound NM23-H1. Overexpression of miR-28-5p reduced CRC cell proliferation, migration, and invasion in vitro, whereas miR-28-3p increased CRC cell migration and invasion in vitro. CRC cells overexpressing miR-28 developed tumors more slowly in mice compared with control cells, but miR-28 promoted tumor metastasis in mice. Conclusion: miR-28-5p and miR-28-3p are transcribed from the same RNA hairpin and are down-regulated in CRC cells. Overexpression of each has different effects on CRC cell proliferation and migration. Such information has a direct application for the design of miRNA gene therapy trials.

Original languageEnglish
JournalGastroenterology
Volume142
Issue number4
DOIs
Publication statusPublished - Apr 2012

Keywords

  • Gene
  • RNA Processing
  • Transcript Regulation

ASJC Scopus subject areas

  • Gastroenterology

Fingerprint Dive into the research topics of 'Strand-specific miR-28-5p and miR-28-3p have distinct effects in colorectal cancer cells'. Together they form a unique fingerprint.

Cite this