Strategies combining cells and scaffolds for bone tissue engineering

E. Saino, L. Fassina, M. S. Sbarra, M. G Cusella De Angelis, G. Magenes, F. Benazzo, L. Visai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Engineering bone typically uses highly porous scaffolds, osteoblasts or cells that can become osteoblasts, and regulating factors that promote cell attachment, differentiation, and mineralized bone formation. In this study we investigated the effects of the electromagnetic stimulation on SAOS-2 cells, from a human osteosarcoma cell line using a sintered 3D titanium scaffold. In comparison with control conditions (standard cell culture incubator, where no electromagnetic stimulus was detectable), the electromagnetic stimulus (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and the surface coating with decorin, osteocalcin, osteopontin, and type-I collagen. The electromagnetic stimulus aimed at obtaining an improved cell proliferation and production of bone proteins, with a consequent surface coating of the scaffold. The protein -coated 3D titanium scaffold could be used, in clinical applications, as an implant for bone repair.

Original languageEnglish
Title of host publicationIFMBE Proceedings
Pages105-108
Number of pages4
Volume25
Edition10
DOIs
Publication statusPublished - 2009
EventWorld Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs - Munich, Germany
Duration: Sep 7 2009Sep 12 2009

Other

OtherWorld Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs
CountryGermany
CityMunich
Period9/7/099/12/09

Fingerprint

Scaffolds (biology)
Tissue engineering
Bone
Osteoblasts
Cell proliferation
Titanium
Scaffolds
Decorin
Proteins
Coatings
Osteopontin
Osteocalcin
Collagen Type I
Cell culture
Collagen
Repair
Cells
Magnetic fields

Keywords

  • Biomimetics
  • Bone extracell ular matrix
  • Cell proliferation
  • Electromagnetic stimulation
  • Osteoblast
  • Surface modification
  • Titanium alloy scaffold

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Saino, E., Fassina, L., Sbarra, M. S., De Angelis, M. G. C., Magenes, G., Benazzo, F., & Visai, L. (2009). Strategies combining cells and scaffolds for bone tissue engineering. In IFMBE Proceedings (10 ed., Vol. 25, pp. 105-108) https://doi.org/10.1007/978-3-642-03900-3-31

Strategies combining cells and scaffolds for bone tissue engineering. / Saino, E.; Fassina, L.; Sbarra, M. S.; De Angelis, M. G Cusella; Magenes, G.; Benazzo, F.; Visai, L.

IFMBE Proceedings. Vol. 25 10. ed. 2009. p. 105-108.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Saino, E, Fassina, L, Sbarra, MS, De Angelis, MGC, Magenes, G, Benazzo, F & Visai, L 2009, Strategies combining cells and scaffolds for bone tissue engineering. in IFMBE Proceedings. 10 edn, vol. 25, pp. 105-108, World Congress on Medical Physics and Biomedical Engineering: Biomaterials, Cellular and Tissue Engineering, Artificial Organs, Munich, Germany, 9/7/09. https://doi.org/10.1007/978-3-642-03900-3-31
Saino E, Fassina L, Sbarra MS, De Angelis MGC, Magenes G, Benazzo F et al. Strategies combining cells and scaffolds for bone tissue engineering. In IFMBE Proceedings. 10 ed. Vol. 25. 2009. p. 105-108 https://doi.org/10.1007/978-3-642-03900-3-31
Saino, E. ; Fassina, L. ; Sbarra, M. S. ; De Angelis, M. G Cusella ; Magenes, G. ; Benazzo, F. ; Visai, L. / Strategies combining cells and scaffolds for bone tissue engineering. IFMBE Proceedings. Vol. 25 10. ed. 2009. pp. 105-108
@inproceedings{b1907654c82c450db04b8484deef7ee3,
title = "Strategies combining cells and scaffolds for bone tissue engineering",
abstract = "Engineering bone typically uses highly porous scaffolds, osteoblasts or cells that can become osteoblasts, and regulating factors that promote cell attachment, differentiation, and mineralized bone formation. In this study we investigated the effects of the electromagnetic stimulation on SAOS-2 cells, from a human osteosarcoma cell line using a sintered 3D titanium scaffold. In comparison with control conditions (standard cell culture incubator, where no electromagnetic stimulus was detectable), the electromagnetic stimulus (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and the surface coating with decorin, osteocalcin, osteopontin, and type-I collagen. The electromagnetic stimulus aimed at obtaining an improved cell proliferation and production of bone proteins, with a consequent surface coating of the scaffold. The protein -coated 3D titanium scaffold could be used, in clinical applications, as an implant for bone repair.",
keywords = "Biomimetics, Bone extracell ular matrix, Cell proliferation, Electromagnetic stimulation, Osteoblast, Surface modification, Titanium alloy scaffold",
author = "E. Saino and L. Fassina and Sbarra, {M. S.} and {De Angelis}, {M. G Cusella} and G. Magenes and F. Benazzo and L. Visai",
year = "2009",
doi = "10.1007/978-3-642-03900-3-31",
language = "English",
isbn = "9783642038990",
volume = "25",
pages = "105--108",
booktitle = "IFMBE Proceedings",
edition = "10",

}

TY - GEN

T1 - Strategies combining cells and scaffolds for bone tissue engineering

AU - Saino, E.

AU - Fassina, L.

AU - Sbarra, M. S.

AU - De Angelis, M. G Cusella

AU - Magenes, G.

AU - Benazzo, F.

AU - Visai, L.

PY - 2009

Y1 - 2009

N2 - Engineering bone typically uses highly porous scaffolds, osteoblasts or cells that can become osteoblasts, and regulating factors that promote cell attachment, differentiation, and mineralized bone formation. In this study we investigated the effects of the electromagnetic stimulation on SAOS-2 cells, from a human osteosarcoma cell line using a sintered 3D titanium scaffold. In comparison with control conditions (standard cell culture incubator, where no electromagnetic stimulus was detectable), the electromagnetic stimulus (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and the surface coating with decorin, osteocalcin, osteopontin, and type-I collagen. The electromagnetic stimulus aimed at obtaining an improved cell proliferation and production of bone proteins, with a consequent surface coating of the scaffold. The protein -coated 3D titanium scaffold could be used, in clinical applications, as an implant for bone repair.

AB - Engineering bone typically uses highly porous scaffolds, osteoblasts or cells that can become osteoblasts, and regulating factors that promote cell attachment, differentiation, and mineralized bone formation. In this study we investigated the effects of the electromagnetic stimulation on SAOS-2 cells, from a human osteosarcoma cell line using a sintered 3D titanium scaffold. In comparison with control conditions (standard cell culture incubator, where no electromagnetic stimulus was detectable), the electromagnetic stimulus (magnetic field, 2 mT; frequency, 75 Hz) increased the cell proliferation and the surface coating with decorin, osteocalcin, osteopontin, and type-I collagen. The electromagnetic stimulus aimed at obtaining an improved cell proliferation and production of bone proteins, with a consequent surface coating of the scaffold. The protein -coated 3D titanium scaffold could be used, in clinical applications, as an implant for bone repair.

KW - Biomimetics

KW - Bone extracell ular matrix

KW - Cell proliferation

KW - Electromagnetic stimulation

KW - Osteoblast

KW - Surface modification

KW - Titanium alloy scaffold

UR - http://www.scopus.com/inward/record.url?scp=77950145513&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950145513&partnerID=8YFLogxK

U2 - 10.1007/978-3-642-03900-3-31

DO - 10.1007/978-3-642-03900-3-31

M3 - Conference contribution

AN - SCOPUS:77950145513

SN - 9783642038990

VL - 25

SP - 105

EP - 108

BT - IFMBE Proceedings

ER -