Strategies for identifying genes that play a role in spinal cord regeneration

M. Wintzer, M. Mladinic, D. Lazarevic, C. Casseler, A. Cattaneo, J. Nicholls

Research output: Contribution to journalArticle

Abstract

A search for genes that promote or block CNS regeneration requires numerous approaches; for example, tests can be made on individual candidate molecules. Here, however, we describe methods for comprehensive identification of genes up- and down-regulated in neurons that can and cannot regenerate after injury. One problem concerns identification of low-abundance genes out of the 30 000 or so genes expressed by neurons. Another difficulty is knowing whether a single gene or multiple genes are necessary. When microchips and subtractive differential display are used to identify genes turned on or off, the numbers are still too great to test which molecules are actually important for regeneration. Candidates are genes coding for trophic, inhibitory, receptor and extracellular matrix molecules, as well as unknown genes. A preparation useful for narrowing the search is the neonatal opossum. The spinal cord and optic nerve can regenerate after injury at 9 days but cannot at 12 days after birth. This narrow window allows genes responsible for the turning off of regeneration to be identified. As a next step, sites at which they are expressed (forebrain, midbrain, spinal cord, neurons or glia, intracellular or extracellular) must be determined. An essential step is to characterize proteins, their levels of expression, and their importance for regeneration. Comprehensive searches for molecular mechanisms represent a lengthy series of experiments that could help in devising strategies for repairing injured spinal cord.

Original languageEnglish
Pages (from-to)3-11
Number of pages9
JournalJournal of Anatomy
Volume204
Issue number1
DOIs
Publication statusPublished - Jan 2004

Fingerprint

Spinal Cord Regeneration
spinal cord
regeneration
gene
Genes
genes
Regeneration
Spinal Cord
neurons
Neurons
brain
Opossums
Spinal Nerves
Wounds and Injuries
opossums
Optic Nerve
Prosencephalon
Mesencephalon
optics
extracellular matrix

Keywords

  • CNS lesions
  • CNS repair
  • Molecular analysis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Strategies for identifying genes that play a role in spinal cord regeneration. / Wintzer, M.; Mladinic, M.; Lazarevic, D.; Casseler, C.; Cattaneo, A.; Nicholls, J.

In: Journal of Anatomy, Vol. 204, No. 1, 01.2004, p. 3-11.

Research output: Contribution to journalArticle

Wintzer, M. ; Mladinic, M. ; Lazarevic, D. ; Casseler, C. ; Cattaneo, A. ; Nicholls, J. / Strategies for identifying genes that play a role in spinal cord regeneration. In: Journal of Anatomy. 2004 ; Vol. 204, No. 1. pp. 3-11.
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