Strategies for quantitation of phosphoproteomic data

Giuseppe Palmisano, Tine E. Thingholm

Research output: Contribution to journalArticle

Abstract

Recent developments in phosphoproteomic sample-preparation techniques and sensitive mass spectrometry instrumentation have led to large-scale identifications of phosphoproteins and phosphorylation sites from highly complex samples. This has facilitated the implementation of different quantitation strategies in order to study the biological role of protein phosphorylation during disease progression, differentiation or during external stimulation of a cellular system. In this article, a brief summary of the most popular strategies for phosphoproteomic studies is given; however, the main focus will be on different quantitation strategies. Methods for metabolic labeling, chemical modification and label-free quantitation and their applicability or inapplicability in phosphoproteomic studies are discussed.

Original languageEnglish
Pages (from-to)439-456
Number of pages18
JournalExpert Review of Proteomics
Volume7
Issue number3
DOIs
Publication statusPublished - Jun 2010

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Keywords

  • Chemical modification
  • ITRAQ
  • Label-free quantitation
  • Mass spectrometry
  • Metabolic labeling
  • Phosphoproteomics
  • Quantitation
  • SILAC
  • Tandem mass tag

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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