Streptokinase induces intravascular release of platelet-activating factor in patients with acute myocardial infarction and stimulates its synthesis by cultured human endothelial cells

G. Montrucchio, S. Bergerone, F. Bussolino, G. Alloatti, L. Silvestro, E. Lupia, A. Cravetto, M. Di Leo, G. Emanuelli, G. Camussi

Research output: Contribution to journalArticle

Abstract

Background. Reocclusion of a successfully recanalized infarct-related artery may account for failure of thrombolytic therapy. Evidence suggests that the intravascular activation of platelets may limit the response to this treatment. The aim of the present study was to investigate whether platelet- activating factor (PAF), an ether lipid mediator with multiple potent biological activities, is synthesized during therapy with thrombolytic agents. Two sets of experiments were performed: (1) we extracted and quantified PAF in blood of patients with acute myocardial infarction treated or untreated with streptokinase (SK), and (2) since the endothelium/platelet interaction is thought to be at the basis of vascular reocclusion, we studied whether cultured human endothelial cells synthesize PAF after stimulation with SK or plasmin. Methods and Results. PAF was extracted from blood samples immediately after acidification to destroy the acid-labile PAF- acetylhydrolase in 25 patients with acute myocardial infarction treated (group A, n=14) and untreated (group B, n=11) with intravenous infusion of SK. PAF was detected in 10 of 14 patients of group A and none of group B. PAF began to be detectable 60 to 90 minutes after SK infusion and disappeared from the circulation within 120 to 180 minutes. Percent variation of platelet count over basal values correlated negatively with the amount of PAF present in the circulation at 90 minutes (r=-.719; P

Original languageEnglish
Pages (from-to)1476-1483
Number of pages8
JournalCirculation
Volume88
Issue number4 I
Publication statusPublished - 1993

Keywords

  • endothelium
  • ischemia
  • platelets
  • thrombolysis

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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    Montrucchio, G., Bergerone, S., Bussolino, F., Alloatti, G., Silvestro, L., Lupia, E., Cravetto, A., Di Leo, M., Emanuelli, G., & Camussi, G. (1993). Streptokinase induces intravascular release of platelet-activating factor in patients with acute myocardial infarction and stimulates its synthesis by cultured human endothelial cells. Circulation, 88(4 I), 1476-1483.