Stress-induced nuclear bodies are sites of accumulation of pre-mRNA processing factors

M. Denegri, I. Chiodi, M. Corioni, F. Cobianchi, S. Riva, G. Biamonti

Research output: Contribution to journalArticle

Abstract

Heterogeneous nuclear ribonucleoprotein (hnRNP) HAP (hnRNP A1 interacting protein) is a multifunctional protein with roles in RNA metabolism, transcription, and nuclear structure. After stress treatments, HAP is recruited to a small number of nuclear bodies, usually adjacent to the nucleoli, which consist of clusters of perichromatin granules and are depots of transcripts synthesized before stress. In this article we show that HAP bodies are sites of accumulation for a subset of RNA processing factors and are related to Sam68 nuclear bodies (SNBs) detectable in unstressed cells. Indeed, HAP and Sam68 are both present in SNBs and in HAP bodies, that we rename "stress-induced SNBs." The determinants required for the redistribution of HAP lie between residue 580 and 788. Different portions of this region direct the recruitment of the green fluorescent protein to stress-induced SNBs, suggesting an interaction of HAP with different components of the bodies. With the use of the 580-725 region as bait in a two-hybrid screening, we have selected SRp30c and 9G8, two members of the SR family of splicing factors. Splicing factors are differentially affected by heat shock: SRp30c and SF2/ASF are efficiently recruited to stress-induced SNBs, whereas the distribution of SC35 is not perturbed. We propose that the differential sequestration of splicing factors could affect processing of specific transcripts. Accordingly, the formation of stress-induced SNBs is accompanied by a change in the splicing pattern of the adenovirus E1A transcripts.

Original languageEnglish
Pages (from-to)3502-3514
Number of pages13
JournalMolecular Biology of the Cell
Volume12
Issue number11
Publication statusPublished - 2001

Fingerprint

RNA Precursors
Heterogeneous-Nuclear Ribonucleoproteins
RNA
Green Fluorescent Proteins
Adenoviridae
hnRNP A1
Shock
Proteins
Hot Temperature
RNA Splicing Factors

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Denegri, M., Chiodi, I., Corioni, M., Cobianchi, F., Riva, S., & Biamonti, G. (2001). Stress-induced nuclear bodies are sites of accumulation of pre-mRNA processing factors. Molecular Biology of the Cell, 12(11), 3502-3514.

Stress-induced nuclear bodies are sites of accumulation of pre-mRNA processing factors. / Denegri, M.; Chiodi, I.; Corioni, M.; Cobianchi, F.; Riva, S.; Biamonti, G.

In: Molecular Biology of the Cell, Vol. 12, No. 11, 2001, p. 3502-3514.

Research output: Contribution to journalArticle

Denegri, M, Chiodi, I, Corioni, M, Cobianchi, F, Riva, S & Biamonti, G 2001, 'Stress-induced nuclear bodies are sites of accumulation of pre-mRNA processing factors', Molecular Biology of the Cell, vol. 12, no. 11, pp. 3502-3514.
Denegri, M. ; Chiodi, I. ; Corioni, M. ; Cobianchi, F. ; Riva, S. ; Biamonti, G. / Stress-induced nuclear bodies are sites of accumulation of pre-mRNA processing factors. In: Molecular Biology of the Cell. 2001 ; Vol. 12, No. 11. pp. 3502-3514.
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