Stress response, survival and enhancement of heat sensitivity in a human melanoma cell line treated with L-canavanine

E. Mattei, D. Damasi, A. M. Mileo, A. Delpino, U. Ferrini

Research output: Contribution to journalArticlepeer-review

Abstract

L-Canavanine, like other aminoacid analogs, induces the synthesis of heat shock proteins (HPSs) but, unlike heat or other stressing agents, it fails to induce thermotolerance. We have studied the synthesis and the intracellular distribution of HSPs induced by canavanine, the effects of this analog on the viability and thermal sensitivity of a human melanoma cell line (M14) and the capacity of canavanine-induced HSPs to self regulate their own synthesis. Evidence indicates that the HSP induction is time- and dose-dependent and, also in the presence of arginine, is not associated with the development of thermotolerance. On the contrary, cells become more heat sensitive and are less efficient in the control of the feed-back mechanism that regulates HSP synthesis. The possible utilization of this substance as a potential aid for the treatment of tumors, in association with heat, was examined.

Original languageEnglish
Pages (from-to)757-762
Number of pages6
JournalAnticancer Research
Volume12
Issue number3
Publication statusPublished - 1992

Keywords

  • Heat shock proteins
  • L-canavanine
  • Melanoma
  • Thermosensitivity

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Fingerprint Dive into the research topics of 'Stress response, survival and enhancement of heat sensitivity in a human melanoma cell line treated with L-canavanine'. Together they form a unique fingerprint.

Cite this