Striatal dopamine metabolism in monoamine oxidase B-deficient mice: A brain dialysis study

Francesco Fornai, Kevin Chen, Filippo Sean Giorgi, Marco Gesi, Maria Grazia Alessandri, Jean Chen Shih

Research output: Contribution to journalArticlepeer-review

Abstract

We have studied striatal dopamine (DA) metabolism in monoamine oxidase (MAO) B-deficient mice using brain microdialysis. Baseline DA levels were similar in wild-type and knock-out (KO) mice. Administration of a selective MAO A inhibitor, clorgyline (2 mg/kg), increased DA levels and decreased levels of its metabolites in all mice, but a selective MAO B inhibitor, l- deprenyl (1 mg/kg), had no effect. Administration of 10 and 50 mg/kg L-DOPA, the precursor of DA, increased the levels of DA similarly in wild-type and KO mice. The highest dose of L-DOPA (100 mg/kg) produced a larger increase in DA in KO than wild-type mice. This difference was abolished by pretreating wild- type mice with l-deprenyl. These results suggest that in mice, DA is only metabolized by MAO A under basal conditions and by both MAO A and B at high concentrations. This is in contrast to the rat, where DA is always metabolized by MAO A regardless of concentration.

Original languageEnglish
Pages (from-to)2434-2440
Number of pages7
JournalJournal of Neurochemistry
Volume73
Issue number6
DOIs
Publication statusPublished - 1999

Keywords

  • Brain dialysis
  • Dopamine
  • L-DOPA
  • Monoamine oxidase
  • Monoamine oxidase B knock-out mice

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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