Striatal dopamine metabolism in monoamine oxidase B-deficient mice: A brain dialysis study

Francesco Fornai, Kevin Chen, Filippo Sean Giorgi, Marco Gesi, Maria Grazia Alessandri, Jean Chen Shih

Research output: Contribution to journalArticle

Abstract

We have studied striatal dopamine (DA) metabolism in monoamine oxidase (MAO) B-deficient mice using brain microdialysis. Baseline DA levels were similar in wild-type and knock-out (KO) mice. Administration of a selective MAO A inhibitor, clorgyline (2 mg/kg), increased DA levels and decreased levels of its metabolites in all mice, but a selective MAO B inhibitor, l- deprenyl (1 mg/kg), had no effect. Administration of 10 and 50 mg/kg L-DOPA, the precursor of DA, increased the levels of DA similarly in wild-type and KO mice. The highest dose of L-DOPA (100 mg/kg) produced a larger increase in DA in KO than wild-type mice. This difference was abolished by pretreating wild- type mice with l-deprenyl. These results suggest that in mice, DA is only metabolized by MAO A under basal conditions and by both MAO A and B at high concentrations. This is in contrast to the rat, where DA is always metabolized by MAO A regardless of concentration.

Original languageEnglish
Pages (from-to)2434-2440
Number of pages7
JournalJournal of Neurochemistry
Volume73
Issue number6
DOIs
Publication statusPublished - 1999

Fingerprint

Corpus Striatum
Dialysis
Monoamine Oxidase
Metabolism
Dopamine
Brain
Knockout Mice
Selegiline
Monoamine Oxidase Inhibitors
Clorgyline
Microdialysis
Metabolites
Rats

Keywords

  • Brain dialysis
  • Dopamine
  • L-DOPA
  • Monoamine oxidase
  • Monoamine oxidase B knock-out mice

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Striatal dopamine metabolism in monoamine oxidase B-deficient mice : A brain dialysis study. / Fornai, Francesco; Chen, Kevin; Giorgi, Filippo Sean; Gesi, Marco; Alessandri, Maria Grazia; Shih, Jean Chen.

In: Journal of Neurochemistry, Vol. 73, No. 6, 1999, p. 2434-2440.

Research output: Contribution to journalArticle

Fornai, Francesco ; Chen, Kevin ; Giorgi, Filippo Sean ; Gesi, Marco ; Alessandri, Maria Grazia ; Shih, Jean Chen. / Striatal dopamine metabolism in monoamine oxidase B-deficient mice : A brain dialysis study. In: Journal of Neurochemistry. 1999 ; Vol. 73, No. 6. pp. 2434-2440.
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