Stringent thiol-mediated retention in B lymphocytes and Xenopus oocytes correlates with inefficient IgM polymerization

Stephana Carelli, Aldo Ceriotti, Roberto Sitia

Research output: Contribution to journalArticle

Abstract

Thiol-dependent retention mechanisms involving the μs chain Cys575 ensure that only polymeric IgM are secreted. B lymphocytes are unable to polymerize IgM and degrade unpolymerized precursors intracellularly. Since several non-lymphoid transfectants secrete hexameric IgM, specific mechanism(s) inhibiting IgM polymerization/secretion may be active in B cells. Here, we show that Xenopus laevis oocytes are also unable to polymerize IgM and retain this isotype via Cys575 as efficiently as B cells. The mechanisms and the hierarchy of the thiol-dependent pre-Golgi retention are conserved in amphibian oocytes, as indicated by the efficient retention of secretory IgA and the slow secretion of unassembled J558 λ chains. We also show that B cells do not lack any structural component necessary to polymerize IgM after retention has been weakened by 2-mercaptoethanol, polymerization can occur if oxidizing conditions are restored. Since release from retention can result in polymerization, stringent retention in B cells and oocytes might be at the basis of their common inability to polymerize secretory IgM. Our findings suggest that disulfide interchange reactions in the exocytic compartment can be modulated during B cell differentiation to control IgM secretion.

Original languageEnglish
Pages (from-to)1283-1291
Number of pages9
JournalEuropean Journal of Immunology
Volume27
Issue number5
Publication statusPublished - Mar 1997

Keywords

  • B cell differentiation
  • Disulfide interchange
  • Endoplasmic reticulum
  • Protein secretion
  • Redox regulation

ASJC Scopus subject areas

  • Immunology

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