Stromal Activation by Tumor Cells

An in Vitro Study in Breast Cancer

Research output: Contribution to journalArticle

Abstract

BACKGROUND: The tumor microenvironment participates in the regulation of tumor progression and influences treatment sensitivity. In breast cancer, it also may play a role in determining the fate of non-invasive lesions such as ductal carcinoma in situ (DCIS), a non-obligate precursor of invasive diseases, which is aggressively treated despite its indolent nature in many patients since no biomarkers are available to predict the progression of DCIS to invasive disease. In vitro models of stromal activation by breast tumor cells might provide clues as to specific stromal genes crucial for the transition from DCIS to invasive disease.

METHODS: normal human dermal fibroblasts (NHDF) were treated under serum-free conditions with cell culture media conditioned by breast cancer cell lines (SkBr3, MDA-MB-468, T47D) for 72 h and subjected to gene expression profiling with Illumina platform.

RESULTS: TGM2, coding for a tissue transglutaminase, was identified as candidate gene for stromal activation. In public transcriptomic datasets of invasive breast tumors TGM2 expression proved to provide prognostic information. Conversely, its role as an early biosensor of tumor invasiveness needs to be further investigated by in situ analyses.

CONCLUSION: Stromal TGM2 might probably be associated with precancerous evolution at earlier stages compared to DCIS.

Original languageEnglish
JournalMicroarrays (Basel, Switzerland)
Volume5
Issue number2
DOIs
Publication statusPublished - May 18 2016

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Carcinoma, Intraductal, Noninfiltrating
Tumors
Chemical activation
Cells
Breast Neoplasms
Neoplasms
Genes
Tumor Microenvironment
Biosensing Techniques
Gene Expression Profiling
Transcriptional Activation
Culture Media
Biomarkers
Fibroblasts
Conditioned Culture Medium
Cell culture
Gene expression
Biosensors
Cell Culture Techniques
Cell Line

Keywords

  • Journal Article

Cite this

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title = "Stromal Activation by Tumor Cells: An in Vitro Study in Breast Cancer",
abstract = "BACKGROUND: The tumor microenvironment participates in the regulation of tumor progression and influences treatment sensitivity. In breast cancer, it also may play a role in determining the fate of non-invasive lesions such as ductal carcinoma in situ (DCIS), a non-obligate precursor of invasive diseases, which is aggressively treated despite its indolent nature in many patients since no biomarkers are available to predict the progression of DCIS to invasive disease. In vitro models of stromal activation by breast tumor cells might provide clues as to specific stromal genes crucial for the transition from DCIS to invasive disease.METHODS: normal human dermal fibroblasts (NHDF) were treated under serum-free conditions with cell culture media conditioned by breast cancer cell lines (SkBr3, MDA-MB-468, T47D) for 72 h and subjected to gene expression profiling with Illumina platform.RESULTS: TGM2, coding for a tissue transglutaminase, was identified as candidate gene for stromal activation. In public transcriptomic datasets of invasive breast tumors TGM2 expression proved to provide prognostic information. Conversely, its role as an early biosensor of tumor invasiveness needs to be further investigated by in situ analyses.CONCLUSION: Stromal TGM2 might probably be associated with precancerous evolution at earlier stages compared to DCIS.",
keywords = "Journal Article",
author = "Giuseppe Merlino and Patrizia Miodini and Biagio Paolini and Carcangiu, {Maria Luisa} and Massimiliano Gennaro and Matteo Dugo and Daidone, {Maria Grazia} and Vera Cappelletti",
year = "2016",
month = "5",
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doi = "10.3390/microarrays5020010",
language = "English",
volume = "5",
journal = "Microarrays",
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TY - JOUR

T1 - Stromal Activation by Tumor Cells

T2 - An in Vitro Study in Breast Cancer

AU - Merlino, Giuseppe

AU - Miodini, Patrizia

AU - Paolini, Biagio

AU - Carcangiu, Maria Luisa

AU - Gennaro, Massimiliano

AU - Dugo, Matteo

AU - Daidone, Maria Grazia

AU - Cappelletti, Vera

PY - 2016/5/18

Y1 - 2016/5/18

N2 - BACKGROUND: The tumor microenvironment participates in the regulation of tumor progression and influences treatment sensitivity. In breast cancer, it also may play a role in determining the fate of non-invasive lesions such as ductal carcinoma in situ (DCIS), a non-obligate precursor of invasive diseases, which is aggressively treated despite its indolent nature in many patients since no biomarkers are available to predict the progression of DCIS to invasive disease. In vitro models of stromal activation by breast tumor cells might provide clues as to specific stromal genes crucial for the transition from DCIS to invasive disease.METHODS: normal human dermal fibroblasts (NHDF) were treated under serum-free conditions with cell culture media conditioned by breast cancer cell lines (SkBr3, MDA-MB-468, T47D) for 72 h and subjected to gene expression profiling with Illumina platform.RESULTS: TGM2, coding for a tissue transglutaminase, was identified as candidate gene for stromal activation. In public transcriptomic datasets of invasive breast tumors TGM2 expression proved to provide prognostic information. Conversely, its role as an early biosensor of tumor invasiveness needs to be further investigated by in situ analyses.CONCLUSION: Stromal TGM2 might probably be associated with precancerous evolution at earlier stages compared to DCIS.

AB - BACKGROUND: The tumor microenvironment participates in the regulation of tumor progression and influences treatment sensitivity. In breast cancer, it also may play a role in determining the fate of non-invasive lesions such as ductal carcinoma in situ (DCIS), a non-obligate precursor of invasive diseases, which is aggressively treated despite its indolent nature in many patients since no biomarkers are available to predict the progression of DCIS to invasive disease. In vitro models of stromal activation by breast tumor cells might provide clues as to specific stromal genes crucial for the transition from DCIS to invasive disease.METHODS: normal human dermal fibroblasts (NHDF) were treated under serum-free conditions with cell culture media conditioned by breast cancer cell lines (SkBr3, MDA-MB-468, T47D) for 72 h and subjected to gene expression profiling with Illumina platform.RESULTS: TGM2, coding for a tissue transglutaminase, was identified as candidate gene for stromal activation. In public transcriptomic datasets of invasive breast tumors TGM2 expression proved to provide prognostic information. Conversely, its role as an early biosensor of tumor invasiveness needs to be further investigated by in situ analyses.CONCLUSION: Stromal TGM2 might probably be associated with precancerous evolution at earlier stages compared to DCIS.

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DO - 10.3390/microarrays5020010

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JO - Microarrays

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