Stromal cell-derived factor-1 as a chemoattractant for follicular center lymphoma B cells

Anna Corcione, Luciano Ottonello, Giuseppe Tortolina, Paola Facchetti, Irma Airoldi, Roberta Guglielmino, Patrizia Dadati, Mauro Truini, Silvano Sozzani, Franco Dallegri, Vito Pistoia

Research output: Contribution to journalArticle

Abstract

Background: Follicular center lymphoma displays widespread lymph node involvement at diagnosis. The chemoattractants that control the locomotion of follicular center lymphoma B cells have not been established. Stromal cell- derived factor-1 (SDF-1) is a CXC-class chemokine that enhances the migration of normal human B cells and is expressed in peripheral lymphoid tissues. Here we have investigated 1) whether SDF-1 stimulates the in vitro locomotion of follicular center lymphoma B cells and of their presumed normal counterparts (i.e., germinal center B cells) and 2) whether the same cells express SDF-1 transcripts. Methods: B cells were purified by immunomagnetic bead manipulation. Messenger RNA was detected by reverse transcription-polymerase chain reaction. Migration was assessed by the filter and collagen invasion assays. All P values were two sided. Results: Follicular center lymphoma B lymphocytes showed a statistically significant migratory response to 300 ng/mL SDF-1, both in the filter and in the collagen assays (P = .002 for each). Such response was mediated by the SDF-1 receptor, CXCR4. CD40 monoclonal antibody (MAb) and tonsillar germinal center B cells treated with CD40 MAb and recombinant interleukin 4, but not freshly isolated, migrated statistically significantly faster in the presence than in the absence of SDF-1 (P = .002 in both filter and collagen assays). Freshly isolated follicular center lymphoma and germinal center B cells expressed SDF-1 transcripts. Conclusions: This study shows that SDF-1 substantially enhances the migration of follicular center lymphoma B cells but not the migration of freshly purified germinal center B cells. This difference may be related to the extended survival of follicular center lymphoma versus germinal center B cells. SDF-1 produced in follicular center lymphoma lymph nodes may play a role in the local dissemination of tumor cells.

Original languageEnglish
Pages (from-to)628-635
Number of pages8
JournalJournal of the National Cancer Institute
Volume92
Issue number8
Publication statusPublished - Apr 19 2000

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Chemokine CXCL12
Follicular Lymphoma
Chemotactic Factors
B-Lymphocytes
Germinal Center
Collagen
Locomotion
Lymph Nodes
Monoclonal Antibodies
CXCR4 Receptors
CXC Chemokines
Lymphoid Tissue
Interleukin-4
Reverse Transcription
Cell Movement

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Corcione, A., Ottonello, L., Tortolina, G., Facchetti, P., Airoldi, I., Guglielmino, R., ... Pistoia, V. (2000). Stromal cell-derived factor-1 as a chemoattractant for follicular center lymphoma B cells. Journal of the National Cancer Institute, 92(8), 628-635.

Stromal cell-derived factor-1 as a chemoattractant for follicular center lymphoma B cells. / Corcione, Anna; Ottonello, Luciano; Tortolina, Giuseppe; Facchetti, Paola; Airoldi, Irma; Guglielmino, Roberta; Dadati, Patrizia; Truini, Mauro; Sozzani, Silvano; Dallegri, Franco; Pistoia, Vito.

In: Journal of the National Cancer Institute, Vol. 92, No. 8, 19.04.2000, p. 628-635.

Research output: Contribution to journalArticle

Corcione, A, Ottonello, L, Tortolina, G, Facchetti, P, Airoldi, I, Guglielmino, R, Dadati, P, Truini, M, Sozzani, S, Dallegri, F & Pistoia, V 2000, 'Stromal cell-derived factor-1 as a chemoattractant for follicular center lymphoma B cells', Journal of the National Cancer Institute, vol. 92, no. 8, pp. 628-635.
Corcione, Anna ; Ottonello, Luciano ; Tortolina, Giuseppe ; Facchetti, Paola ; Airoldi, Irma ; Guglielmino, Roberta ; Dadati, Patrizia ; Truini, Mauro ; Sozzani, Silvano ; Dallegri, Franco ; Pistoia, Vito. / Stromal cell-derived factor-1 as a chemoattractant for follicular center lymphoma B cells. In: Journal of the National Cancer Institute. 2000 ; Vol. 92, No. 8. pp. 628-635.
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AU - Corcione, Anna

AU - Ottonello, Luciano

AU - Tortolina, Giuseppe

AU - Facchetti, Paola

AU - Airoldi, Irma

AU - Guglielmino, Roberta

AU - Dadati, Patrizia

AU - Truini, Mauro

AU - Sozzani, Silvano

AU - Dallegri, Franco

AU - Pistoia, Vito

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N2 - Background: Follicular center lymphoma displays widespread lymph node involvement at diagnosis. The chemoattractants that control the locomotion of follicular center lymphoma B cells have not been established. Stromal cell- derived factor-1 (SDF-1) is a CXC-class chemokine that enhances the migration of normal human B cells and is expressed in peripheral lymphoid tissues. Here we have investigated 1) whether SDF-1 stimulates the in vitro locomotion of follicular center lymphoma B cells and of their presumed normal counterparts (i.e., germinal center B cells) and 2) whether the same cells express SDF-1 transcripts. Methods: B cells were purified by immunomagnetic bead manipulation. Messenger RNA was detected by reverse transcription-polymerase chain reaction. Migration was assessed by the filter and collagen invasion assays. All P values were two sided. Results: Follicular center lymphoma B lymphocytes showed a statistically significant migratory response to 300 ng/mL SDF-1, both in the filter and in the collagen assays (P = .002 for each). Such response was mediated by the SDF-1 receptor, CXCR4. CD40 monoclonal antibody (MAb) and tonsillar germinal center B cells treated with CD40 MAb and recombinant interleukin 4, but not freshly isolated, migrated statistically significantly faster in the presence than in the absence of SDF-1 (P = .002 in both filter and collagen assays). Freshly isolated follicular center lymphoma and germinal center B cells expressed SDF-1 transcripts. Conclusions: This study shows that SDF-1 substantially enhances the migration of follicular center lymphoma B cells but not the migration of freshly purified germinal center B cells. This difference may be related to the extended survival of follicular center lymphoma versus germinal center B cells. SDF-1 produced in follicular center lymphoma lymph nodes may play a role in the local dissemination of tumor cells.

AB - Background: Follicular center lymphoma displays widespread lymph node involvement at diagnosis. The chemoattractants that control the locomotion of follicular center lymphoma B cells have not been established. Stromal cell- derived factor-1 (SDF-1) is a CXC-class chemokine that enhances the migration of normal human B cells and is expressed in peripheral lymphoid tissues. Here we have investigated 1) whether SDF-1 stimulates the in vitro locomotion of follicular center lymphoma B cells and of their presumed normal counterparts (i.e., germinal center B cells) and 2) whether the same cells express SDF-1 transcripts. Methods: B cells were purified by immunomagnetic bead manipulation. Messenger RNA was detected by reverse transcription-polymerase chain reaction. Migration was assessed by the filter and collagen invasion assays. All P values were two sided. Results: Follicular center lymphoma B lymphocytes showed a statistically significant migratory response to 300 ng/mL SDF-1, both in the filter and in the collagen assays (P = .002 for each). Such response was mediated by the SDF-1 receptor, CXCR4. CD40 monoclonal antibody (MAb) and tonsillar germinal center B cells treated with CD40 MAb and recombinant interleukin 4, but not freshly isolated, migrated statistically significantly faster in the presence than in the absence of SDF-1 (P = .002 in both filter and collagen assays). Freshly isolated follicular center lymphoma and germinal center B cells expressed SDF-1 transcripts. Conclusions: This study shows that SDF-1 substantially enhances the migration of follicular center lymphoma B cells but not the migration of freshly purified germinal center B cells. This difference may be related to the extended survival of follicular center lymphoma versus germinal center B cells. SDF-1 produced in follicular center lymphoma lymph nodes may play a role in the local dissemination of tumor cells.

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