TY - JOUR
T1 - Stromal cells and human malignant neuroblasts derived from bone marrow metastasis may share common karyotypic abnormalities
T2 - The case of the IGR-N-91 cell line
AU - Raschell, Giuseppe
AU - Tanno, Barbara
AU - Bonetto, Francesco
AU - Negroni, Anna
AU - Amendola, Roberto
AU - Paggi, Marco G.
PY - 2001
Y1 - 2001
N2 - Background. Stage IV neuroblastoma is characterized by tumor invasion and metastatic dissemination. Cell lines derived from such neuroblastomas have a high in vitro proliferation capacity. Procedure. We established three neuroblastoma cell lines derived from involved bone marrow of three patients with stage IV neuroblastoma and performed a cytogenetic study. Results. Various culture conditions allowed us to distinguish two cell subpopulations: Malignant neuroblasts (Nb-type) and substrate-adherent stromal cells (Str-type). Karyotypic analyses revealed two specific chromosomal abnormalities in diploid malignant IGR-N-331 neuroblasts, der(1)t(1;7)(p22;q11) and der(5)t(5;17)(q35;q21), one unbalanced translocation der(1)t(1;17)(p35;q21)×2 in hyperdipioid malignant IGR-N-337 neuroblasts, and a normal karyotype in both corresponding stromal subpopulations. In contrast, in the IGR-N-91 model, both cell types shared two unbalanced translocations, t(1;4)(q12;p15) and t(2;10)(p14;q11), suggesting that stromal cells and malignant neuroblasts originate from a common stem cell. Conclusions. Based on our findings, we postulate that genetically modified stromal cells may influence the metastatic potential of malignant neuroblasts.
AB - Background. Stage IV neuroblastoma is characterized by tumor invasion and metastatic dissemination. Cell lines derived from such neuroblastomas have a high in vitro proliferation capacity. Procedure. We established three neuroblastoma cell lines derived from involved bone marrow of three patients with stage IV neuroblastoma and performed a cytogenetic study. Results. Various culture conditions allowed us to distinguish two cell subpopulations: Malignant neuroblasts (Nb-type) and substrate-adherent stromal cells (Str-type). Karyotypic analyses revealed two specific chromosomal abnormalities in diploid malignant IGR-N-331 neuroblasts, der(1)t(1;7)(p22;q11) and der(5)t(5;17)(q35;q21), one unbalanced translocation der(1)t(1;17)(p35;q21)×2 in hyperdipioid malignant IGR-N-337 neuroblasts, and a normal karyotype in both corresponding stromal subpopulations. In contrast, in the IGR-N-91 model, both cell types shared two unbalanced translocations, t(1;4)(q12;p15) and t(2;10)(p14;q11), suggesting that stromal cells and malignant neuroblasts originate from a common stem cell. Conclusions. Based on our findings, we postulate that genetically modified stromal cells may influence the metastatic potential of malignant neuroblasts.
KW - Cytogenetics
KW - Neuroblastoma
KW - Stromal cells
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U2 - 10.1002/1096-911X(20010101)36:1<100::AID-MPO1023>3.0.CO;2-G
DO - 10.1002/1096-911X(20010101)36:1<100::AID-MPO1023>3.0.CO;2-G
M3 - Article
C2 - 11464856
AN - SCOPUS:0035171137
VL - 36
SP - 100
EP - 103
JO - Pediatric Blood and Cancer
JF - Pediatric Blood and Cancer
SN - 1545-5009
IS - 1
ER -