Stromal cells from human decidua exert a strong inhibitory effect on NK cell function and dendritic cell differentiation

Daniele Croxatto, Paola Vacca, Francesca Canegallo, Romana Conte, Pier Luigi Venturini, Lorenzo Moretta, Maria Cristina Mingari

Research output: Contribution to journalArticle

Abstract

Stromal cells (SC) are an important component of decidual tissues where they are in strict proximity with both NK and CD14+ myelomonocytic cells that play a role in the maintenance of pregnancy. In this study we analyzed whether decidual SC (DSC) could exert a regulatory role on NK and CD14+ cells that migrate from peripheral blood (PB) to decidua during pregnancy. We show that DSCs inhibit the IL15-mediated up-regulation of major activating NK receptors in PB-derived NK cells. In addition, the IL15-induced NK cell proliferation, cytolytic activity and IFN-γ production were severely impaired. DSCs sharply inhibited dendritic cells differentiation and their ability to induce allogeneic T cell proliferation. Indoleamine 2,3-dioxygenase (IDO) and prostaglandin E2 (PGE2) mediated the inhibitory effect of DSCs. Our results strongly suggest an important role of DSCs in preventing potentially dangerous immune response, thus contributing to maintenance of pregnancy.

Original languageEnglish
Article numbere89006
JournalPLoS One
Volume9
Issue number2
DOIs
Publication statusPublished - Feb 20 2014

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Interleukin-15
Decidua
stromal cells
natural killer cells
Cell proliferation
dendritic cells
Stromal Cells
cell differentiation
Natural Killer Cells
Pregnancy Maintenance
Dendritic Cells
Cell Differentiation
Blood
pregnancy
Indoleamine-Pyrrole 2,3,-Dioxygenase
T-cells
cell proliferation
Dinoprostone
Cell Proliferation
blood

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Stromal cells from human decidua exert a strong inhibitory effect on NK cell function and dendritic cell differentiation. / Croxatto, Daniele; Vacca, Paola; Canegallo, Francesca; Conte, Romana; Venturini, Pier Luigi; Moretta, Lorenzo; Mingari, Maria Cristina.

In: PLoS One, Vol. 9, No. 2, e89006, 20.02.2014.

Research output: Contribution to journalArticle

Croxatto, Daniele ; Vacca, Paola ; Canegallo, Francesca ; Conte, Romana ; Venturini, Pier Luigi ; Moretta, Lorenzo ; Mingari, Maria Cristina. / Stromal cells from human decidua exert a strong inhibitory effect on NK cell function and dendritic cell differentiation. In: PLoS One. 2014 ; Vol. 9, No. 2.
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