Stromal damage as consequence of high-dose chemo/radiotherapy in bone marrow transplant recipients

Maria Galotto, Giovanni Berisso, Laura Delfino, Marina Podesta, Laura Ottaggio, Sandro Dallorso, Carlo Dufour, Giovanni Battista Ferrara, Angelo Abbondandolo, Giorgio Dini, Andrea Bacigalupo, Ranieri Cancedda, Rodolfo Quarto

Research output: Contribution to journalArticle

Abstract

Bone marrow transplant (BMT) relies on the engraftment of donor hemopoietic precursors in the host marrow space. Colony forming units- fibroblasts (CFU-f), the precursor compartment for the osteogenic lineage, are essential to hemopoietic stem cell survival, proliferation and differentiation. We have studied CFU-f in donors (aged 5 months to 62 years) and in patients who had received allogeneic BMT (aged 2 months to 63 years). In donor marrows we found an inverse correlation between CFU-f frequency and age. In BMT recipients CFU-f frequencies were reduced by 60%-90% (p <0.05) and the numbers did not recover up to 12 years after transplant. Stromal reconstitution to normal levels was found only in patients <5 years old. In all patients studied CFU-f post-BMT were of host origin. Patients with low CFU-f levels displayed also a decreased bone mineral density (p <0.05) and significantly reduced levels of long-term culture-initiating cells (LTC-IC) (p <0.05). Our study demonstrates that the marrow stromal microenvironment is seriously and irreversibly damaged after BMT. Donor cells do not contribute to reconstitute the marrow microenvironment, whose residual CFU-fs remain of host origin.

Original languageEnglish
Pages (from-to)1460-1466
Number of pages7
JournalExperimental Hematology
Volume27
Issue number9
DOIs
Publication statusPublished - Sep 1999

Keywords

  • Bone marrow transplant
  • CFU-f
  • LTC-IC

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

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  • Cite this

    Galotto, M., Berisso, G., Delfino, L., Podesta, M., Ottaggio, L., Dallorso, S., Dufour, C., Ferrara, G. B., Abbondandolo, A., Dini, G., Bacigalupo, A., Cancedda, R., & Quarto, R. (1999). Stromal damage as consequence of high-dose chemo/radiotherapy in bone marrow transplant recipients. Experimental Hematology, 27(9), 1460-1466. https://doi.org/10.1016/S0301-472X(99)00076-4