Stromal derived factor-1α (SDF-1α) induces CD4+ T cell apoptosis via the functional up-regulation of the Fas (CD95)/Fas ligand (CD95L) pathway

Maria Luisa Colamussi, Paola Secchiero, Arianna Gonelli, Marco Marchisio, Giorgio Zauli, Silvano Capitani

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Stromal-derived factor-1α (SDF-1α), the high-affinity ligand of CXC-chemokine receptor 4 (CXCR4), induced a progressive increase of apoptosis when added to the Jurkat CD4+/CXCR4+ T cell line. The SDF-1α-mediated Jurkat cell apoptosis was observed in serum-free or serum-containing cultures, peaked at SDF-1α concentrations of 10-100 ng/ml, required 3 days to take place, and was completely blocked by the z-VAD-fmk tripeptide caspase inhibitor. Although SDF-1α did not modify the expression of TNF-α or that of TNF-RI and TNF-RII, it increased the expression of surface Fas/APO-1 (CD95) and intracellular Fas ligand (CD95L) significantly. Moreover, the ability of SDF-1α to induce apoptosis was inhibited by an anti-CD95 Fab' neutralizing antibody. These findings suggest a role for SDF-1α in the homeostatic control of CD4+ T-cell survival/apoptosis mediated by the CD95-CD95L pathway.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalJournal of Leukocyte Biology
Volume69
Issue number2
Publication statusPublished - 2001

Fingerprint

Fas Ligand Protein
Up-Regulation
Apoptosis
T-Lymphocytes
CXCR4 Receptors
Receptors, Tumor Necrosis Factor, Type II
Caspase Inhibitors
Jurkat Cells
Neutralizing Antibodies
Serum
Cell Survival
Ligands
Cell Line

Keywords

  • CXCR4
  • T lymphocytes
  • TNF-α
  • TNF-RI
  • TNF-RII

ASJC Scopus subject areas

  • Cell Biology

Cite this

Colamussi, M. L., Secchiero, P., Gonelli, A., Marchisio, M., Zauli, G., & Capitani, S. (2001). Stromal derived factor-1α (SDF-1α) induces CD4+ T cell apoptosis via the functional up-regulation of the Fas (CD95)/Fas ligand (CD95L) pathway. Journal of Leukocyte Biology, 69(2), 263-270.

Stromal derived factor-1α (SDF-1α) induces CD4+ T cell apoptosis via the functional up-regulation of the Fas (CD95)/Fas ligand (CD95L) pathway. / Colamussi, Maria Luisa; Secchiero, Paola; Gonelli, Arianna; Marchisio, Marco; Zauli, Giorgio; Capitani, Silvano.

In: Journal of Leukocyte Biology, Vol. 69, No. 2, 2001, p. 263-270.

Research output: Contribution to journalArticle

Colamussi, ML, Secchiero, P, Gonelli, A, Marchisio, M, Zauli, G & Capitani, S 2001, 'Stromal derived factor-1α (SDF-1α) induces CD4+ T cell apoptosis via the functional up-regulation of the Fas (CD95)/Fas ligand (CD95L) pathway', Journal of Leukocyte Biology, vol. 69, no. 2, pp. 263-270.
Colamussi, Maria Luisa ; Secchiero, Paola ; Gonelli, Arianna ; Marchisio, Marco ; Zauli, Giorgio ; Capitani, Silvano. / Stromal derived factor-1α (SDF-1α) induces CD4+ T cell apoptosis via the functional up-regulation of the Fas (CD95)/Fas ligand (CD95L) pathway. In: Journal of Leukocyte Biology. 2001 ; Vol. 69, No. 2. pp. 263-270.
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