Abstract
Stromal-derived factor-1α (SDF-1α), the high-affinity ligand of CXC-chemokine receptor 4 (CXCR4), induced a progressive increase of apoptosis when added to the Jurkat CD4+/CXCR4+ T cell line. The SDF-1α-mediated Jurkat cell apoptosis was observed in serum-free or serum-containing cultures, peaked at SDF-1α concentrations of 10-100 ng/ml, required 3 days to take place, and was completely blocked by the z-VAD-fmk tripeptide caspase inhibitor. Although SDF-1α did not modify the expression of TNF-α or that of TNF-RI and TNF-RII, it increased the expression of surface Fas/APO-1 (CD95) and intracellular Fas ligand (CD95L) significantly. Moreover, the ability of SDF-1α to induce apoptosis was inhibited by an anti-CD95 Fab' neutralizing antibody. These findings suggest a role for SDF-1α in the homeostatic control of CD4+ T-cell survival/apoptosis mediated by the CD95-CD95L pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 263-270 |
| Number of pages | 8 |
| Journal | Journal of Leukocyte Biology |
| Volume | 69 |
| Issue number | 2 |
| Publication status | Published - 2001 |
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Keywords
- CXCR4
- T lymphocytes
- TNF-α
- TNF-RI
- TNF-RII
ASJC Scopus subject areas
- Cell Biology
Cite this
Stromal derived factor-1α (SDF-1α) induces CD4+ T cell apoptosis via the functional up-regulation of the Fas (CD95)/Fas ligand (CD95L) pathway. / Colamussi, Maria Luisa; Secchiero, Paola; Gonelli, Arianna; Marchisio, Marco; Zauli, Giorgio; Capitani, Silvano.
In: Journal of Leukocyte Biology, Vol. 69, No. 2, 2001, p. 263-270.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stromal derived factor-1α (SDF-1α) induces CD4+ T cell apoptosis via the functional up-regulation of the Fas (CD95)/Fas ligand (CD95L) pathway
AU - Colamussi, Maria Luisa
AU - Secchiero, Paola
AU - Gonelli, Arianna
AU - Marchisio, Marco
AU - Zauli, Giorgio
AU - Capitani, Silvano
PY - 2001
Y1 - 2001
N2 - Stromal-derived factor-1α (SDF-1α), the high-affinity ligand of CXC-chemokine receptor 4 (CXCR4), induced a progressive increase of apoptosis when added to the Jurkat CD4+/CXCR4+ T cell line. The SDF-1α-mediated Jurkat cell apoptosis was observed in serum-free or serum-containing cultures, peaked at SDF-1α concentrations of 10-100 ng/ml, required 3 days to take place, and was completely blocked by the z-VAD-fmk tripeptide caspase inhibitor. Although SDF-1α did not modify the expression of TNF-α or that of TNF-RI and TNF-RII, it increased the expression of surface Fas/APO-1 (CD95) and intracellular Fas ligand (CD95L) significantly. Moreover, the ability of SDF-1α to induce apoptosis was inhibited by an anti-CD95 Fab' neutralizing antibody. These findings suggest a role for SDF-1α in the homeostatic control of CD4+ T-cell survival/apoptosis mediated by the CD95-CD95L pathway.
AB - Stromal-derived factor-1α (SDF-1α), the high-affinity ligand of CXC-chemokine receptor 4 (CXCR4), induced a progressive increase of apoptosis when added to the Jurkat CD4+/CXCR4+ T cell line. The SDF-1α-mediated Jurkat cell apoptosis was observed in serum-free or serum-containing cultures, peaked at SDF-1α concentrations of 10-100 ng/ml, required 3 days to take place, and was completely blocked by the z-VAD-fmk tripeptide caspase inhibitor. Although SDF-1α did not modify the expression of TNF-α or that of TNF-RI and TNF-RII, it increased the expression of surface Fas/APO-1 (CD95) and intracellular Fas ligand (CD95L) significantly. Moreover, the ability of SDF-1α to induce apoptosis was inhibited by an anti-CD95 Fab' neutralizing antibody. These findings suggest a role for SDF-1α in the homeostatic control of CD4+ T-cell survival/apoptosis mediated by the CD95-CD95L pathway.
KW - CXCR4
KW - T lymphocytes
KW - TNF-α
KW - TNF-RI
KW - TNF-RII
UR - http://www.scopus.com/inward/record.url?scp=0035109871&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035109871&partnerID=8YFLogxK
M3 - Article
C2 - 11272277
AN - SCOPUS:0035109871
VL - 69
SP - 263
EP - 270
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
SN - 0741-5400
IS - 2
ER -