Stromal derived factor-1α (SDF-1α) induces CD4+ T cell apoptosis via the functional up-regulation of the Fas (CD95)/Fas ligand (CD95L) pathway

Maria Luisa Colamussi, Paola Secchiero, Arianna Gonelli, Marco Marchisio, Giorgio Zauli, Silvano Capitani

Research output: Contribution to journalArticlepeer-review

Abstract

Stromal-derived factor-1α (SDF-1α), the high-affinity ligand of CXC-chemokine receptor 4 (CXCR4), induced a progressive increase of apoptosis when added to the Jurkat CD4+/CXCR4+ T cell line. The SDF-1α-mediated Jurkat cell apoptosis was observed in serum-free or serum-containing cultures, peaked at SDF-1α concentrations of 10-100 ng/ml, required 3 days to take place, and was completely blocked by the z-VAD-fmk tripeptide caspase inhibitor. Although SDF-1α did not modify the expression of TNF-α or that of TNF-RI and TNF-RII, it increased the expression of surface Fas/APO-1 (CD95) and intracellular Fas ligand (CD95L) significantly. Moreover, the ability of SDF-1α to induce apoptosis was inhibited by an anti-CD95 Fab' neutralizing antibody. These findings suggest a role for SDF-1α in the homeostatic control of CD4+ T-cell survival/apoptosis mediated by the CD95-CD95L pathway.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalJournal of Leukocyte Biology
Volume69
Issue number2
Publication statusPublished - 2001

Keywords

  • CXCR4
  • T lymphocytes
  • TNF-α
  • TNF-RI
  • TNF-RII

ASJC Scopus subject areas

  • Cell Biology

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