Structural analysis of the epitope of the anti-HIV antibody 2F5 sheds light into its mechanism of neutralization and HIV fusion

Gaetano Barbato, Elisabetta Bianchi, Paolo Ingallinella, William H. Hurni, Michael D. Miller, Gennaro Ciliberto, Riccardo Cortese, Renzo Bazzo, John W. Shiver, Antonello Pessi

Research output: Contribution to journalArticle

121 Citations (Scopus)

Abstract

Inhibition of human immunodeficiency virus (HIV) fusion with the host cell has emerged as a viable therapeutic strategy, and rational design of inhibitors and vaccines, interfering with this process, is a prime target for antiviral research. To advance our knowledge of the structural biology of HIV fusion, we have studied the membrane-proximal region of the fusogenic envelope subunit gp41, which includes the epitope ELDKWA of the broadly neutralizing human antibody 2F5. The structural evidence available for this region is contradictory, with some studies suggesting an overall helical conformation, while the X-ray structure of the ELDKWAS peptide bound to the antibody shows it folded in a type I β turn. We used a two-step strategy: Firstly, by a competition binding assay, we identified the proper boundaries of the domain recognized by 2F5, which we found considerably larger than the ELDKWAS hexapeptide. Secondly, we studied the structure of the resulting 13 amino acid residue peptide by collecting NMR data and analyzing them by our previously developed statistical method (NAMFIS). Our study revealed that the increase in binding affinity goes in parallel with stabilization of specific local and global conformational propensities, absent from the shorter epitope. When compounded with the available biological evidence, our structural analysis allows us to propose a specific role for the membrane-proximal region during HIV fusion, in terms of a conformational transition between the turn and the helical structure. At the same time, our hypothesis offers a structural explanation for the mechanism of neutralization of mAb 2F5.

Original languageEnglish
Pages (from-to)1101-1115
Number of pages15
JournalJournal of Molecular Biology
Volume330
Issue number5
DOIs
Publication statusPublished - Jul 25 2003

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Epitopes
HIV
Antibodies
Peptides
Membranes
Neutralizing Antibodies
Antiviral Agents
Vaccines
X-Rays
Amino Acids
Research
Therapeutics

Keywords

  • 2F5 epitope
  • gp41
  • HIV-1 fusion
  • NMR spectroscopy
  • Statistical conformational analysis

ASJC Scopus subject areas

  • Virology

Cite this

Structural analysis of the epitope of the anti-HIV antibody 2F5 sheds light into its mechanism of neutralization and HIV fusion. / Barbato, Gaetano; Bianchi, Elisabetta; Ingallinella, Paolo; Hurni, William H.; Miller, Michael D.; Ciliberto, Gennaro; Cortese, Riccardo; Bazzo, Renzo; Shiver, John W.; Pessi, Antonello.

In: Journal of Molecular Biology, Vol. 330, No. 5, 25.07.2003, p. 1101-1115.

Research output: Contribution to journalArticle

Barbato, G, Bianchi, E, Ingallinella, P, Hurni, WH, Miller, MD, Ciliberto, G, Cortese, R, Bazzo, R, Shiver, JW & Pessi, A 2003, 'Structural analysis of the epitope of the anti-HIV antibody 2F5 sheds light into its mechanism of neutralization and HIV fusion', Journal of Molecular Biology, vol. 330, no. 5, pp. 1101-1115. https://doi.org/10.1016/S0022-2836(03)00611-9
Barbato, Gaetano ; Bianchi, Elisabetta ; Ingallinella, Paolo ; Hurni, William H. ; Miller, Michael D. ; Ciliberto, Gennaro ; Cortese, Riccardo ; Bazzo, Renzo ; Shiver, John W. ; Pessi, Antonello. / Structural analysis of the epitope of the anti-HIV antibody 2F5 sheds light into its mechanism of neutralization and HIV fusion. In: Journal of Molecular Biology. 2003 ; Vol. 330, No. 5. pp. 1101-1115.
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AU - Miller, Michael D.

AU - Ciliberto, Gennaro

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AB - Inhibition of human immunodeficiency virus (HIV) fusion with the host cell has emerged as a viable therapeutic strategy, and rational design of inhibitors and vaccines, interfering with this process, is a prime target for antiviral research. To advance our knowledge of the structural biology of HIV fusion, we have studied the membrane-proximal region of the fusogenic envelope subunit gp41, which includes the epitope ELDKWA of the broadly neutralizing human antibody 2F5. The structural evidence available for this region is contradictory, with some studies suggesting an overall helical conformation, while the X-ray structure of the ELDKWAS peptide bound to the antibody shows it folded in a type I β turn. We used a two-step strategy: Firstly, by a competition binding assay, we identified the proper boundaries of the domain recognized by 2F5, which we found considerably larger than the ELDKWAS hexapeptide. Secondly, we studied the structure of the resulting 13 amino acid residue peptide by collecting NMR data and analyzing them by our previously developed statistical method (NAMFIS). Our study revealed that the increase in binding affinity goes in parallel with stabilization of specific local and global conformational propensities, absent from the shorter epitope. When compounded with the available biological evidence, our structural analysis allows us to propose a specific role for the membrane-proximal region during HIV fusion, in terms of a conformational transition between the turn and the helical structure. At the same time, our hypothesis offers a structural explanation for the mechanism of neutralization of mAb 2F5.

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