Structural and functional features of the α3 chain indicate a bridging role for chicken collagen VI in connective tissues

Paolo Bonaldo, Vincenzo Russo, Francesco Bucciotti, Roberto Doliana, Alfonso Colombatti

Research output: Contribution to journalArticle

Abstract

Type VI collagen is a component of 100 nm long periodic filaments with a widespread distribution around collagen fibers and on the surface of cells. It is an unusual collagen constituted by three distinct chains, one of which (α3) is much larger than the others and is encoded by a 9-kb mRNA. The amino acid sequence of the α3(VI) deduced from the present cDNA clones specifies for a multidomain protein of at least 2648 residues made of a short collagenous sequence (336 residues), flanked at the N-terminus by nine 200 residue long repeating motifs and at the C-terminus by two similar motifs that share extensive identities with the collagen-binding type A repeats of von Willebrand factor. Type VI collagen and α3(VI) fusion proteins bound to insolubilized type I collagen in a specific, time-dependent, and saturable manner. The α3(VI) chain has three Arg-Gly-Asp sequences in the collagenous domain, and cell attachment was stimulated by the triple helix of type VI collagen and by α3(VI) fusion proteins containing Arg-Gly-Asp sequences. This function was specifically inhibited by the Arg-Gly-Asp-Ser synthetic peptide. The type 1 collagen-binding and the cell-attachment properties of the α3(VI) chain provide direct information for the role of type VI collagen in connective tissues.

Original languageEnglish
Pages (from-to)1245-1254
Number of pages10
JournalBiochemistry
Volume29
Issue number5
Publication statusPublished - 1990

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Structural and functional features of the α3 chain indicate a bridging role for chicken collagen VI in connective tissues'. Together they form a unique fingerprint.

  • Cite this

    Bonaldo, P., Russo, V., Bucciotti, F., Doliana, R., & Colombatti, A. (1990). Structural and functional features of the α3 chain indicate a bridging role for chicken collagen VI in connective tissues. Biochemistry, 29(5), 1245-1254.