Structural and functional modifications induced by ketamine on synaptosomes in the rat

Laura Mazzanti, G. Lenaz, F. Marinelli, S. Cinti

Research output: Contribution to journalArticle

Abstract

This study correlates previous observations on the effects of a general anaesthetic (2-chlorophenyl-2-methylaminocyclohexanone, trade name Ketalar), administered in vivo on the functional properties of synaptosomes from the brain of the rat, with structural modifications detected using freeze-fracture electron microscopy. The anaesthetized rats exhibited a fluidization of the synaptic membranes, as probed by stearic acid spin labels, accompanied by a decrease in the activity of acetylcholinesterase. The freeze-fracture images of synaptosomes from anaesthetized rats showed a clear statistical increase in the number of vesicles with aggregated intramembrane particles, without any significant change of the particle diameter. The hypothesis that a perturbation of the lipid protein interaction is a primary effect, in the mechanism of action of anaesthetics, is supported at present only by indirect evidence.

Original languageEnglish
Pages (from-to)1343-1349
Number of pages7
JournalNeuropharmacology
Volume30
Issue number12
DOIs
Publication statusPublished - 1991

Fingerprint

Synaptosomes
Ketamine
Spin Labels
Synaptic Membranes
General Anesthetics
Acetylcholinesterase
Names
Anesthetics
Electron Microscopy
Lipids
Brain
Proteins

Keywords

  • acetylcholinesterase
  • enzymatic activity
  • freeze-fracturing
  • ketamine
  • membranes
  • rat synaptosomes

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

Structural and functional modifications induced by ketamine on synaptosomes in the rat. / Mazzanti, Laura; Lenaz, G.; Marinelli, F.; Cinti, S.

In: Neuropharmacology, Vol. 30, No. 12, 1991, p. 1343-1349.

Research output: Contribution to journalArticle

Mazzanti, Laura ; Lenaz, G. ; Marinelli, F. ; Cinti, S. / Structural and functional modifications induced by ketamine on synaptosomes in the rat. In: Neuropharmacology. 1991 ; Vol. 30, No. 12. pp. 1343-1349.
@article{fa4d22f1fdf24754a9d37d5e5fe5ef35,
title = "Structural and functional modifications induced by ketamine on synaptosomes in the rat",
abstract = "This study correlates previous observations on the effects of a general anaesthetic (2-chlorophenyl-2-methylaminocyclohexanone, trade name Ketalar), administered in vivo on the functional properties of synaptosomes from the brain of the rat, with structural modifications detected using freeze-fracture electron microscopy. The anaesthetized rats exhibited a fluidization of the synaptic membranes, as probed by stearic acid spin labels, accompanied by a decrease in the activity of acetylcholinesterase. The freeze-fracture images of synaptosomes from anaesthetized rats showed a clear statistical increase in the number of vesicles with aggregated intramembrane particles, without any significant change of the particle diameter. The hypothesis that a perturbation of the lipid protein interaction is a primary effect, in the mechanism of action of anaesthetics, is supported at present only by indirect evidence.",
keywords = "acetylcholinesterase, enzymatic activity, freeze-fracturing, ketamine, membranes, rat synaptosomes",
author = "Laura Mazzanti and G. Lenaz and F. Marinelli and S. Cinti",
year = "1991",
doi = "10.1016/0028-3908(91)90032-7",
language = "English",
volume = "30",
pages = "1343--1349",
journal = "Neuropharmacology",
issn = "0028-3908",
publisher = "Elsevier Limited",
number = "12",

}

TY - JOUR

T1 - Structural and functional modifications induced by ketamine on synaptosomes in the rat

AU - Mazzanti, Laura

AU - Lenaz, G.

AU - Marinelli, F.

AU - Cinti, S.

PY - 1991

Y1 - 1991

N2 - This study correlates previous observations on the effects of a general anaesthetic (2-chlorophenyl-2-methylaminocyclohexanone, trade name Ketalar), administered in vivo on the functional properties of synaptosomes from the brain of the rat, with structural modifications detected using freeze-fracture electron microscopy. The anaesthetized rats exhibited a fluidization of the synaptic membranes, as probed by stearic acid spin labels, accompanied by a decrease in the activity of acetylcholinesterase. The freeze-fracture images of synaptosomes from anaesthetized rats showed a clear statistical increase in the number of vesicles with aggregated intramembrane particles, without any significant change of the particle diameter. The hypothesis that a perturbation of the lipid protein interaction is a primary effect, in the mechanism of action of anaesthetics, is supported at present only by indirect evidence.

AB - This study correlates previous observations on the effects of a general anaesthetic (2-chlorophenyl-2-methylaminocyclohexanone, trade name Ketalar), administered in vivo on the functional properties of synaptosomes from the brain of the rat, with structural modifications detected using freeze-fracture electron microscopy. The anaesthetized rats exhibited a fluidization of the synaptic membranes, as probed by stearic acid spin labels, accompanied by a decrease in the activity of acetylcholinesterase. The freeze-fracture images of synaptosomes from anaesthetized rats showed a clear statistical increase in the number of vesicles with aggregated intramembrane particles, without any significant change of the particle diameter. The hypothesis that a perturbation of the lipid protein interaction is a primary effect, in the mechanism of action of anaesthetics, is supported at present only by indirect evidence.

KW - acetylcholinesterase

KW - enzymatic activity

KW - freeze-fracturing

KW - ketamine

KW - membranes

KW - rat synaptosomes

UR - http://www.scopus.com/inward/record.url?scp=0026008620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026008620&partnerID=8YFLogxK

U2 - 10.1016/0028-3908(91)90032-7

DO - 10.1016/0028-3908(91)90032-7

M3 - Article

C2 - 1664918

AN - SCOPUS:0026008620

VL - 30

SP - 1343

EP - 1349

JO - Neuropharmacology

JF - Neuropharmacology

SN - 0028-3908

IS - 12

ER -