Abstract
Background: Erythrocyte membrane modifications in patients with cholestasis are supposed to reflect those of hepatocytes. Methods: Erythrocyte membrane composition (cholesterol, phospholipids, fatty acids, protein sulphydrils and carbonyls) was assessed and related to the stage of liver disease in patients with primary biliary cirrhosis before and after 1 year of ursodeoxycholate treatment. Results: Compared with controls, patients showed lower levels of protein sulphydrils (28.9 ± 7.1 vs. 65.6 ± 1.8 nmol mg-1 prot) and accumulation of carbonyls (4.7 ± 1.7 vs. 1.4 ± 0.1 nmol mg-1 prot). Phosphatidylethanolamine level was lower in stage III-IV cirrhosis while phosphatidylcholine and cholesterol levels were higher; as a consequence the phosphatidylcholine/sphingomyelin ratio was higher than in controls (4.25 ± 0.55 in the I-II stage and 2. 89 ± 0.44 in the stage III-IV vs. 1.61 ± 0.30). These changes were particularly evident in patients with more advanced stages of liver disease. Protein sulphydrils and carbonyls, phosphatidylethanolamine and cholesterol levels correlated (P <0.05) with the histological stage of the liver disease, serum and membrane cholesterol levels were significantly related (r = 0.66, P <0.05). One year of ursodeoxycholate administration was accompanied by major changes of the membrane lipid composition, partial reversal of protein oxidation, and improvement of serum parameters. Conclusions: This study indicates that major alterations in protein status and lipid composition occur in erythrocyte membrane of patients with primary biliary cirrhosis. These changes were more pronounced in patients with advanced liver disease. Ursodeoxycholate was able to revert in part serum and erythrocyte alterations, especially in patients with early stages of liver disease.
| Original language | English |
|---|---|
| Pages (from-to) | 868-874 |
| Number of pages | 7 |
| Journal | European Journal of Clinical Investigation |
| Volume | 33 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 1 2003 |
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Keywords
- Cholesterol
- Chronic cholestasis
- Erythrocyte ghost
- Phospholipids
- Primary biliary cirrhosis
- Protein oxidation
ASJC Scopus subject areas
- Medicine(all)
Cite this
Structural and oxidative modifications of erythrocyte ghosts in patients with primary biliary cirrhosis : Relation with the disease stage and effect of bile acid treatment. / Grattagliano, I.; Giudetti, A. M.; Grattagliano, V.; Palmieri, V. O.; Gnoni, G. V.; Lapadula, G.; Palasciano, G.; Vendemiale, Gianluigi.
In: European Journal of Clinical Investigation, Vol. 33, No. 10, 01.10.2003, p. 868-874.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Structural and oxidative modifications of erythrocyte ghosts in patients with primary biliary cirrhosis
T2 - Relation with the disease stage and effect of bile acid treatment
AU - Grattagliano, I.
AU - Giudetti, A. M.
AU - Grattagliano, V.
AU - Palmieri, V. O.
AU - Gnoni, G. V.
AU - Lapadula, G.
AU - Palasciano, G.
AU - Vendemiale, Gianluigi
PY - 2003/10/1
Y1 - 2003/10/1
N2 - Background: Erythrocyte membrane modifications in patients with cholestasis are supposed to reflect those of hepatocytes. Methods: Erythrocyte membrane composition (cholesterol, phospholipids, fatty acids, protein sulphydrils and carbonyls) was assessed and related to the stage of liver disease in patients with primary biliary cirrhosis before and after 1 year of ursodeoxycholate treatment. Results: Compared with controls, patients showed lower levels of protein sulphydrils (28.9 ± 7.1 vs. 65.6 ± 1.8 nmol mg-1 prot) and accumulation of carbonyls (4.7 ± 1.7 vs. 1.4 ± 0.1 nmol mg-1 prot). Phosphatidylethanolamine level was lower in stage III-IV cirrhosis while phosphatidylcholine and cholesterol levels were higher; as a consequence the phosphatidylcholine/sphingomyelin ratio was higher than in controls (4.25 ± 0.55 in the I-II stage and 2. 89 ± 0.44 in the stage III-IV vs. 1.61 ± 0.30). These changes were particularly evident in patients with more advanced stages of liver disease. Protein sulphydrils and carbonyls, phosphatidylethanolamine and cholesterol levels correlated (P <0.05) with the histological stage of the liver disease, serum and membrane cholesterol levels were significantly related (r = 0.66, P <0.05). One year of ursodeoxycholate administration was accompanied by major changes of the membrane lipid composition, partial reversal of protein oxidation, and improvement of serum parameters. Conclusions: This study indicates that major alterations in protein status and lipid composition occur in erythrocyte membrane of patients with primary biliary cirrhosis. These changes were more pronounced in patients with advanced liver disease. Ursodeoxycholate was able to revert in part serum and erythrocyte alterations, especially in patients with early stages of liver disease.
AB - Background: Erythrocyte membrane modifications in patients with cholestasis are supposed to reflect those of hepatocytes. Methods: Erythrocyte membrane composition (cholesterol, phospholipids, fatty acids, protein sulphydrils and carbonyls) was assessed and related to the stage of liver disease in patients with primary biliary cirrhosis before and after 1 year of ursodeoxycholate treatment. Results: Compared with controls, patients showed lower levels of protein sulphydrils (28.9 ± 7.1 vs. 65.6 ± 1.8 nmol mg-1 prot) and accumulation of carbonyls (4.7 ± 1.7 vs. 1.4 ± 0.1 nmol mg-1 prot). Phosphatidylethanolamine level was lower in stage III-IV cirrhosis while phosphatidylcholine and cholesterol levels were higher; as a consequence the phosphatidylcholine/sphingomyelin ratio was higher than in controls (4.25 ± 0.55 in the I-II stage and 2. 89 ± 0.44 in the stage III-IV vs. 1.61 ± 0.30). These changes were particularly evident in patients with more advanced stages of liver disease. Protein sulphydrils and carbonyls, phosphatidylethanolamine and cholesterol levels correlated (P <0.05) with the histological stage of the liver disease, serum and membrane cholesterol levels were significantly related (r = 0.66, P <0.05). One year of ursodeoxycholate administration was accompanied by major changes of the membrane lipid composition, partial reversal of protein oxidation, and improvement of serum parameters. Conclusions: This study indicates that major alterations in protein status and lipid composition occur in erythrocyte membrane of patients with primary biliary cirrhosis. These changes were more pronounced in patients with advanced liver disease. Ursodeoxycholate was able to revert in part serum and erythrocyte alterations, especially in patients with early stages of liver disease.
KW - Cholesterol
KW - Chronic cholestasis
KW - Erythrocyte ghost
KW - Phospholipids
KW - Primary biliary cirrhosis
KW - Protein oxidation
UR - http://www.scopus.com/inward/record.url?scp=0141593582&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141593582&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2362.2003.01238.x
DO - 10.1046/j.1365-2362.2003.01238.x
M3 - Article
C2 - 14511358
AN - SCOPUS:0141593582
VL - 33
SP - 868
EP - 874
JO - European Journal of Clinical Investigation
JF - European Journal of Clinical Investigation
SN - 0014-2972
IS - 10
ER -