Structural and regulatory roles of muscle ankyrin repeat protein family in skeletal muscle

Ilona A. Barash, Marie Louise Bang, Liby Mathew, Marion L. Greaser, Ju Chen, Richard L. Lieber

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The biological response of muscle to eccentric contractions (ECs) results in strengthening and protection from further injury. However, the cellular basis for this response remains unclear. Previous studies identified the muscle ankyrin repeat protein (MARP) family, consisting of cardiac ankyrin repeat protein (CARP), ankyrin repeat domain 2/ankyrin repeat protein with PEST and proline-rich region (Ankrd2/Arpp), and diabetes-associated ankyrin repeat protein (DARP), as rapidly and specifically upregulated in mice after a single bout of EC. To determine the role of these genes in skeletal muscle, a survey of skeletal muscle structural and functional characteristics was performed on mice lacking all three MARP family members (MKO). There was a slight trend toward MKO muscles having a slower fiber type distribution but no differences in muscle fiber size. Single MKO fibers were less stiff, tended to have longer resting sarcomere lengths, and expressed a longer isoform of titin than their wild-type counterparts, indicating that these proteins may play a role in the passive mechanical behavior of muscle. Finally, MKO mice showed a greater degree of torque loss after a bout of ECs compared with wild-type mice, although they recovered from the injury with the same or even improved time course. This recovery was associated with enhanced expression of the muscle regulatory genes MyoD and muscle LIM protein (MLP), suggesting that the MARP family may play both important structural and gene regulatory roles in skeletal muscle.

Original languageEnglish
JournalAmerican Journal of Physiology - Cell Physiology
Volume293
Issue number1
DOIs
Publication statusPublished - Jul 2007

Fingerprint

Ankyrin Repeat
Muscle
Skeletal Muscle
Muscles
Proteins
Regulator Genes
Genes
Connectin
Fibers
Sarcomeres
Wounds and Injuries
Torque
Proline
Protein Isoforms
Medical problems

Keywords

  • Ankrd2
  • Arpp
  • CARP
  • DARP
  • Eccentric contractions
  • Titin

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Structural and regulatory roles of muscle ankyrin repeat protein family in skeletal muscle. / Barash, Ilona A.; Bang, Marie Louise; Mathew, Liby; Greaser, Marion L.; Chen, Ju; Lieber, Richard L.

In: American Journal of Physiology - Cell Physiology, Vol. 293, No. 1, 07.2007.

Research output: Contribution to journalArticle

Barash, Ilona A. ; Bang, Marie Louise ; Mathew, Liby ; Greaser, Marion L. ; Chen, Ju ; Lieber, Richard L. / Structural and regulatory roles of muscle ankyrin repeat protein family in skeletal muscle. In: American Journal of Physiology - Cell Physiology. 2007 ; Vol. 293, No. 1.
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